Total phenolic contents and antioxidant scavenging capacity in free and bound extracts of Christia vespertilionisis (daun rerama)

Mat Jusoh, Hanapi and Zulkarnail, Mohamad Zharif and Haron, Normah (2019) Total phenolic contents and antioxidant scavenging capacity in free and bound extracts of Christia vespertilionisis (daun rerama). International Journal of Allied Health Sciences, 3 (2). pp. 649-657. E-ISSN 2600-8491

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Abstract

Introduction:Christia vespertilionisis is a plant herb that has been used traditionally due to its benefits for potentially to treat some disease. It contain potential phytochemical compound that can be a component of drugs or natural products. This study was conducted to investigate the total phenolic content and its antioxidant radical scavenging activity on C. vespertilionisis. Hence, a series of test in determining the total phenolic content in three different type of extractions was conducted and its relationship with antioxidant scavenging activity was studied. The C. vespertilionis’s powder was extracted with 80% methanol and hydrolyzed with HCI and NaOH. The total phenolic content was determined using Follin-Ciocalteu and DPPH assay was used for antioxidant activity. Based on the test, the total phenolic content of all extracts was 9.13± 0.13 mg of GAE/g with insoluble bound extracts revealed a highest value of 4.94 ± 0.042 mg of GAE/g followed by soluble bound and free phenolic extracts with (3.16± 0.28 and 0.98 ± 0.07 mg of GAE/g respectively), (p<0.05). The antioxidant scavenging capacity extracts of IC50 showed that insoluble bound extracts showed lowest value (38.13 ± 0.65mg/ml) followed by soluble bound extracts and free phenolic extracts with (39.54 ± 0.77 and 42.59 ± 1.69mg/ml respectively).

Item Type:	Article (Journal)
Additional Information:	4588/70263
Uncontrolled Keywords:	Christia Vespertilionisis, total phenolic content, antioxidant scavenging activity, DPPH assay, free phenolic, soluble bound phenolic, insoluble bound phenolic.
Subjects:	Q Science > Q Science (General) Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology Kulliyyah of Allied Health Sciences Kulliyyah of Allied Health Sciences > Department of Nutrition Sciences Kulliyyah of Science
Depositing User:	Dr. Normah Haron
Date Deposited:	11 Sep 2019 15:23
Last Modified:	11 Sep 2019 15:23
URI:	http://irep.iium.edu.my/id/eprint/70263

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