Protein improvement of banana peel through sequential solid state fermentation using mixed-culture of phanerochaete chrysosporium and Candida utilis

Olorunnisola, Kola Saheed and Jamal, Parveen and Alam, Md Zahangir (2018) Protein improvement of banana peel through sequential solid state fermentation using mixed-culture of phanerochaete chrysosporium and Candida utilis. 3 Biotech, 8 (10). pp. 1-10. ISSN 2190-572X

Preview	PDF (SCOPUS) - Supplemental Material Download (204kB) | Preview
Preview	PDF (WOS) - Supplemental Material Download (289kB) | Preview
	PDF - Published Version Restricted to Registered users only Download (1MB) | Request a copy

Abstract

Banana peel (BP) is a major waste produced by fruit processing industries. Pre-treatment of BP at different temperatures led to 40% reduction in saponin at 100 °C (from 9.5 to 5.7 mg/g). Sequential mixed culture of Phanerochaete chrysosporium (P. chrysosporium) and Candida utilis (C. utilis) gave highest protein enrichment (88.93 mg/g). There is 26% increase in protein synthesis (from 88.93 to 111.78 mg/g) after media screening. Inclusion of KH2PO4, FeSO4·7H2O, wheat flour and sucrose in the media contributed positively to protein synthesis, while elevated concentration of urea, peptone, K2HPO4, KCl, NH4H2PO4, and MgSO4.7H2O are required to reach optimum protein synthesis. Total soluble sugar (TSS), total reducing sugar (TRS) and total carbohydrate (CHO) consumption varied with respect to protein synthesis in all experimental runs. Optimum protein synthesis required 6 days and inclusion of 5% sucrose, 0.6% NH4H2PO4, 0.4% KCl, and 0.5% MgSO4·7H2O as concentration media constituents to reach 140.95 mg/g protein synthesis equivalent to 300% increase over the raw banana peel protein content (35.0 mg/g). © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.

Item Type:	Article (Journal)
Additional Information:	2937/70250
Uncontrolled Keywords:	Banana peel, Candida utilis, Phanerochaete chrysosporium, Protein, Solid state fermentation
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof.Dr. Parveen Jamal
Date Deposited:	25 Feb 2019 13:53
Last Modified:	14 Oct 2019 09:13
URI:	http://irep.iium.edu.my/id/eprint/70250

Actions (login required)

View Item