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Quantitative paper-based detection of male fertility biomarkers

Abdul Rahman, Suzanah and Mohd Zahri, Syazana and Abdul Majid, Zafri Azran and Ahmad, Nur Amalina and Abdul Samat, Nadia Hanis and Abdullah, Afif Raihan (2018) Quantitative paper-based detection of male fertility biomarkers. International Journal of Research in Pharmaceutical Sciences, 9 (Special Issue 2). pp. 17-26. E-ISSN 0975-7538

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Abstract

Male infertility is a reproductive disorder culminating from hormonal imbalances which may be caused by stress and use of health supplements. Invasive diagnostic tests and privacy issues have relatively deterred men from seeking infertility treatment. A rapid and reliable test using non-invasive samples is sought to observe the reproductive effects of subchronic thymoquinone administration and prolonged artificial light exposure to rats. Detection of testosterone as the male fertility biomarker from non-invasive samples was employed using adult male Sprague-Dawley rats treated with corticosterone (10 mg/kg) for 10 successive days, followed by a single dose of cisplatin (10 mg/kg), prior to the day of sacrifice. All interventions were given within a 56 days period and sampling of blood, saliva and urine were performed at day 0 and day 56. Testosterone and corticosterone levels were determined using enzyme-linked immunosorbent assay (ELISA). Sperm analysis parameters subsequent to testes harvest at day 56 were measured and followed by a histological assessment. Paper-based lateral flow assay (PLFA) strip was developed based on the colour change from the antigen-antibody reaction on paper reflecting testosterone levels from urine samples. The colour change was recorded using a smartphone camera by an application that captures the RGB colour value. This study demonstrated that 30mg/kg subchronic thymoquinone supplementation can reduce testosterone levels thus possibly affecting fertility. Meanwhile, the 24-hour light exposure showed no significant effects compared to controls. The testosterone level assessment using the PLFA produced comparable data with ELISA results. This sensor holds potential to increase patient compliance with sampling by using non-invasive samples to test infertility in men.

Item Type: Article (Journal)
Additional Information: 4493/69878
Uncontrolled Keywords: Biosensor,Corticosterone,Male Infertility,Paper-based Lateral Flow Assay,RGB Values,Smartphone Application,Testosterone,Thymoquinone
Subjects: Q Science > Q Science (General)
T Technology > TP Chemical technology > TP248.13 Biotechnology
T Technology > TR Photography > TR510 Color photography
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences
Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Kulliyyah of Allied Health Sciences > Department of Diagnostic Imaging and Radiotherapy
Depositing User: Assoc. Prof. Dr. Suzanah Abdul Rahman
Date Deposited: 19 Feb 2019 12:26
Last Modified: 12 Jul 2019 16:43
URI: http://irep.iium.edu.my/id/eprint/69878

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