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Identification and quantification of quercetin, a major constituent of Artocarpus altilis by targeting related genes of apoptosis and cell cycle: in vitro cytotoxic activity against human lung carcinoma cell lines

Jalal, Tara Kamal and Firus Khan, Al'aina Yuhainis and Natto, Hatim A. and Abdull Rasad, Mohammad Syaiful Bahari and Kaderi, Mohd Arifin and Mohammad, Mardhiah and Johan, Muhammad Farid and Omar, Muhammad Nor and Abdul Wahab, Ridhwan (2019) Identification and quantification of quercetin, a major constituent of Artocarpus altilis by targeting related genes of apoptosis and cell cycle: in vitro cytotoxic activity against human lung carcinoma cell lines. Nutrition and Cancer. pp. 1-14. ISSN 0163-5581 E-ISSN 1532-7914 (In Press)

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Abstract

Nine phenolic compounds were identified and quantified in Artocarpus altilia fruit. One of the main compounds was quercetin, which is the major class of flavonoids has been identified and quantified in pulp part of A. altilis fruit of methanol extract. The aim of this study was to evaluate in vitro cytotoxic assay. Inhibitory concentration 50% concentration was determined using trypan blue exclusion assay. Apoptosis induction and cell cycle regulation were studied by flow cytometric analysis. The expression of apoptosis and cell cycle-related regulatory genes were assessed by RT-qPCR study of the methanol extract of pulp part on human lung carcinoma (A549) cell line. A significant increase of cells at G2/M phases was detected (P<0.05). Furthermore, the pulp of the fruit downregulated the expression of antiapoptosis gene BCL-2 and upregulated the expression of pro-apoptosis gene BAX. CASPASE3 was also activated by the fruit, which started a CASPASE-3-depended mitochondrial pathway to induce apoptosis. As the results, the pulp was the most active in terms of all tests, due to high amount of quercetin in pulp part, 78% of total flavonoids. Taken together, these findings suggested that A. altilis induces apoptosis in a mitochondrial-dependent pathway by releasing and upregulating CYTOCHROME C expression and regulates the expression of downstream apoptotic components, including BCL-2 and BAX.

Item Type: Article (Journal)
Additional Information: 4701/69643
Uncontrolled Keywords: quercetin, Artocarpus altilis, carcinoma cell lines, apoptosis, cell cycle
Subjects: R Medicine > RA Public aspects of medicine > RA644.3 Public Health. Hygiene. Preventive Medicine - Chronic and Noninfectious Diseases and Public Health
R Medicine > RM Therapeutics. Pharmacology > RM147 Administration of Drugs and Other Therapeutic Agents
R Medicine > RM Therapeutics. Pharmacology > RM300 Drugs and their action
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences
Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Kulliyyah of Science
Kulliyyah of Science > Department of Biotechnology
Depositing User: Dr Ridhwan Abdul Wahab
Date Deposited: 26 Feb 2019 14:41
Last Modified: 31 Jul 2019 12:17
URI: http://irep.iium.edu.my/id/eprint/69643

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