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Alkaloids from alphonsea elliptica barks and their biological activities

Obaid, Ahmed Kareem and Syed Abdul Azziz, Saripah Salbiah and Mhd Bakri, Yuhanis and Nafiah, Mohd Azlan and Awang, Khalijah and Abdulla Aowda, Saadon and Litaudon, Marc and Mohd Hassan, Norazian and Naz, Humera and Abbas, Phirdaous and Hashim, Yumi Zuhanis Has-Yun and Majhool, Alhussein Arkan (2018) Alkaloids from alphonsea elliptica barks and their biological activities. Journal of Global Pharma Technology, 10 (8). pp. 270-275. ISSN 0975-8542

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Abstract

This study was carried out to investigate the phytochemicals from the barks of Alphonsea elliptica. The barks were harvested from Hutan Simpan Sembarong, Kluang, Johor; and extracted using dichloromethane (DCM), hexane and methanol successively. Isolation of dichloromethane crude extract was then performed using silica gel column chromatography and preparative thin layer chromatography (PTLC) techniques. Structural identification were carried out via 1D and 2D NMR, UV, IR, MS and comparison with reported data. Phytochemical study of the barks led to the isolation of five alkaloid in which two were oxoaporphines; atherospermidine (1) and liriodenine (2),two were dioxoaporphines;cepharadione A (3) and N-methylouregidione (4), and an azafluorenone alkaloid; kinabaline (5). The hexane, DCM, and methanol crude extracts, together with five isolated alkaloids, were tested for their radical scavenging activity, inhibition of xanthine oxidase activities and cytotoxicity. Atherospermidine and liriodenine possessed antioxidative activities with IC50 value of 20.17 and 10.73, respectively. Atherospermidine, liriodenine, N-methylouregidione and kinabaline showed xanthine oxidase inhibitiory activityof 46.29, 7.66, 42.10 and 50.72 μM,respectively. Meanwhile, atherospermidine, liriodenine, cepharadione A and kinabaline showed cytotoxicity against breast cancer cell line with IC50 89, 86, 79.85 and 62 μg/mL respectively. All the alkaloids tested in this study were isolated for the first time from this species while cepharadione A is reported for the first time in a species belongs to Alphonsea genus.

Item Type: Article (Journal)
Additional Information: 4171/68590
Uncontrolled Keywords: Alphonsea elliptica Hk. f. et Th., alkaloids, radical scavenging activity, xanthine oxidase, cytotoxicity.
Subjects: Q Science > QD Chemistry
R Medicine > RS Pharmacy and materia medica > RS403 Materia Medica-Pharmaceutical Chemistry
Kulliyyahs/Centres/Divisions/Institutes: International Institute for Halal Research and Training (INHART)
Kulliyyah of Engineering
Kulliyyah of Engineering > Department of Biotechnology Engineering
Kulliyyah of Pharmacy
Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User: Dr. Norazian Mohd Hassan
Date Deposited: 18 Dec 2018 09:10
Last Modified: 12 Feb 2019 16:33
URI: http://irep.iium.edu.my/id/eprint/68590

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