Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry

Ramli, Nurul Izzati and Abd-Wahab, Firdaus and Wan Salim, Wan Wardatul Amani (2018) Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry. In: 3rd International Sciences, Technology & Engineering Conference (ISTEC) 2018 - Material Chemistry, 18th April 2018, Penang, Malaysia.

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Abstract

Existing practice of glucose-level monitoring for diabetic patients involves frequent finger pricking for blood samples. This is not only constantly painful, but could make patients susceptible to infections. A non-invasive, portable, graphene-based biosensor is being developed to measure glucose levels in saliva instead of in blood, by incorporating rGO-PEDOT:PSS composites as the transducer layer on a working electrode – a screen-printed carbon electrode (SPCE) 2 mm in diameter. To evaluate the performance of the biosensor, the electrochemical technique of linear sweep voltammetry (LSV) was used. The results showed sensitivity of rGO-PEDOT:PSS-GOx/SPCE in artificial saliva: 105 μA/mM (σ = 12.52) at no glucose to 0.2 mM glucose concentrations and 35.33 μA/mM (σ = 5.79) at glucose concentrations of 0.2 mM to 0.6 mM, with correlation coefficient of 0.99. The sensitivity of the biosensor in 10 mM PBS (pH 6.8) is 9.88 μA/mM (σ = 2.74), while in 100 mM potassium ferricyanide it is 10.48 μA/mM (σ = 0.49). The high sensitivity obtained in artificial saliva suggests that the rGO-PEDOT:PSS composite is suitable for use as a transducer layer for non-invasive glucose monitoring.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	7116/68443
Uncontrolled Keywords:	linear sweep voltammetry, screen printed carbon electrode, reduced graphene oxide, PEDOT:PSS, potassium ferricyanide, artificial saliva
Subjects:	T Technology > TP Chemical technology > TP155 Chemical engineering T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Wan Wardatul Amani Wan Salim
Date Deposited:	17 Dec 2018 14:18
Last Modified:	17 Dec 2018 14:18
URI:	http://irep.iium.edu.my/id/eprint/68443

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