Antioxidative and cytotoxic activities of crude and isolated compounds of Polyalthia lateriflora (Bl.) King

Obaid, Ahmed Kareem and Syed Abdul Azziz, Saripah Salbiah and Mhd Bakri, Yuhanis and Nafiah, Mohd Azlan and Awang, Khalijah and Abdulla Aowda, Saadon and Mohd Hassan, Norazian and Hashim, Yumi Zuhanis Has-Yun and Abdullah, Fauziah (2018) Antioxidative and cytotoxic activities of crude and isolated compounds of Polyalthia lateriflora (Bl.) King. Journal of Pharmaceutical Sciences and Research, 10 (11). pp. 2718-2721. ISSN 0975-1459

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Abstract

This study aimed to isolate compounds from Polyalthia lateriflora (Bl.) King stem bark, and to test the crude extracts and the isolated compounds for their antioxidant and cytotoxic activities. Three different solvents were used to prepare the crude extracts; hexane, dichloromethane (DCM) and methanol. Phytochemical investigation of the crude extracts has led to the isolation of four oxoaporphine alkaloids (O-methylmoschatoline, atherospermidine, lysicamine, and liriodenine) and two steroids (stigmasterol and lupeol). The chemical structure of the compounds was elucidated using 1H-NMR, 13C-NMR, 2D-NMR, and mass spectral analysis. All the crude extracts and isolated compounds were tested for their in vitro DPPH radicals scavenging activity and cytotoxicity against MCF-7 breast cancer cells. Methanol extract showed the strongest antioxidant activity with percentage inhibition at 500μg/mL of 89.52±1.81 (IC50, 128.22± 6.73 μg/ml) in comparison with DCM (32.45±8.56), atherospermidine (20.17±9.74) and liriodenine (10.73±0.45), while other extracts were inactive. All the isolated compounds showed cytotoxic against MCF-7 cells, at IC50 values in the range of 42-89 μg/ml. Based on thorough literature review, all compounds identified in this study were isolated for the first time from this plant. In particular, lupeol is reported for the first time in the genus Polyalthia and possessed the most potent cytotoxic effect compared to other isolated compounds.

Item Type:	Article (Journal)
Additional Information:	4171/68245
Uncontrolled Keywords:	Polyalthia lateriflora (Bl.) King, antioxidant, DPPH scavenger, cytotoxicity, MCF-7 cells
Subjects:	Q Science > QD Chemistry R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART) Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr. Norazian Mohd Hassan
Date Deposited:	05 Dec 2018 09:54
Last Modified:	09 Jan 2019 09:01
URI:	http://irep.iium.edu.my/id/eprint/68245

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