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Determination of ultra-morphological changes of trichophyton rubrum treated with hydroxychavicol

P.M, Ridzuan and Mohamad, Nasir and Ismail, Salwani and A. Rahman, Nor Iza and Khalili R, Mohd Adzim and Hamzah, Hairul Aini and Buyong, Zunariah and Mohd Hassan, Norazian and Roesnitac, Baharudin (2016) Determination of ultra-morphological changes of trichophyton rubrum treated with hydroxychavicol. The International Medical Journal Malaysia. ISSN 1823-4631

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Introduction: Trichophyton rubrum is a common pathogenic fungal species that is responsible for causing infection of human skin, hair and nail. The antifungal-resistant strains however, complicate the treatment regime. Hydroxychavicol (HC) is one of the main compounds from the Piper betel leaf that have antifungal potential and its mechanism of action has yet to be studied. This study was carried out to determine the antifungal properties of HC against Trichophyton rubrum using transmission electron microscope (TEM) on gross and ultrastructure of Trichophyton rubrum hypha. Methods: Broth dilution method was used to determine the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of HC and miconazole (MI) against the Trichophyton rubrum (ATCC 28188). Trichophyton rubrum was treated with HC and MI at concentrations of 1.25, 2.5, 5 and 10 mg/mL for 1, 3, 5 and 7 days continuously. Results: The MIC results of the HC and MI against Trichophyton rubrum were 0.00048 mg/mL and 0.000061 mg/mL respectively. MFC results showed 0.0019 mg/mL for HC and 0.000061 mg/mL for MI. Microscopically, the fungi structures became more severely damaged at increasing treatment duration. The cell wall of the fungi treated with HC showed a rough surface, shrinkage and demolition similar to that of the MI treated group. The fungi organelles were also demolished and disorganized. Conclusions: This study reveals that HC has the ability to inhibit Trichophyton rubrum growth and it has the potential to be an antifungal agent especially in treating dermatitis. KEYWORDS: Piper betel, Trichophyton rubrum, hydroxychavicol, miconazole, antifungal

Item Type: Article (Meeting Abstract)
Additional Information: 68239/5342
Uncontrolled Keywords: Piper betel, Trichophyton rubrum, hydroxychavicol, miconazole, antifungal
Subjects: R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Medicine > Department of Basic Medical
Kulliyyah of Pharmacy
Date Deposited: 24 Jan 2019 19:16
Last Modified: 24 Jan 2019 19:16
URI: http://irep.iium.edu.my/id/eprint/68239

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