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Antioxidant activities of Dialium indum L. fruit and gas chromatography-mass spectrometry (GC-MS) of the active fractions

Osman, Muhamad Faris and Mohd Hassan, Norazian and Khatib, Alfi and Tolos, Siti Marponga (2018) Antioxidant activities of Dialium indum L. fruit and gas chromatography-mass spectrometry (GC-MS) of the active fractions. Antioxidants, 7 (11). pp. 1-14. ISSN 2076-3921

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Abstract

The fruit of Dialium indum L. (Fabaceae) is one of the edible wild fruits native to Southeast Asia. The mesocarp is consumed as sweets while the exocarp and seed are regarded as waste. This study aimed to evaluate the antioxidant activities of the fruit by using four assays, which measure its capabilities in reducing phosphomolybdic-phosphotungstic acid reagents, neocuproine, 2,2-diphenyl-picrylhydrazyl (DPPH), and inhibiting linoleic acid peroxidation. The active fractions were then analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that the seed methanol fraction (SMF) exhibited the strongest antioxidant activity with significantly higher (p < 0.05) gallic acid equivalence (GAE), total antioxidant capacity (TAC), and DPPH radical scavenging activity (IC50 31.71; 0.88 μg/mL) than the other fractions. The exocarp dichloromethane fraction (EDF) was the discriminating fraction by having remarkable linoleic acid peroxidation inhibition (IC50 121.43; 2.97 μg/mL). A total of thirty-eight metabolites were detected in derivatized EDF and SMF with distinctive classes of phenolics and amino acids, respectively. Bioautography-guided fractionation of EDF afforded five antioxidant-enriched subfractions with four other detected phenolics. The results revealed the antioxidant properties of D. indum fruit, which has potential benefits in pharmaceutical, nutraceutical, and cosmeceutical applications.

Item Type: Article (Journal)
Additional Information: 4171/67382
Uncontrolled Keywords: Dialium indum; exocarp; seed; antioxidant; phenolic acids; amino acids; GC-MS analysis
Subjects: R Medicine > RS Pharmacy and materia medica > RS403 Materia Medica-Pharmaceutical Chemistry
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Pharmacy
Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Kulliyyah of Science
Kulliyyah of Science > Department of Computational and Theoretical Sciences
Depositing User: Dr. Norazian Mohd Hassan
Date Deposited: 09 Nov 2018 09:53
Last Modified: 31 Dec 2018 20:37
URI: http://irep.iium.edu.my/id/eprint/67382

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