Sequencing-based analysis for the identification of bacteria associated with severe early childhood caries (SECC)

Zainuddin, Zarina and Paiman, Abdul Qahhar and Dewi Ardini, Yunita (2018) Sequencing-based analysis for the identification of bacteria associated with severe early childhood caries (SECC). International Medical Journal Malaysia, 17 (Special Issue 2). pp. 131-135. ISSN 1823-4631

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Abstract

Severe early childhood caries (SECC) is a microbialinfection that severely compromises the dentition ofyoung children.The aim of this study is toidentify bacteria associated with SECC and compare bacterial species between SECC and caries-free children. Bacterial samples were isolated from dental plaque samples of 3 to 6-yearold preschool children and the status of dental caries at the surface level was evaluated using World Health Organization (WHO) diagnostic criteria for decayed, missing and filled tooth surfaces (DFMS). 30 samples from the interproximal part were collected and genomic DNA was extracted using MasterPure™ Gram Positive DNA Purification Kit. Polymerase Reaction (PCR) was conducted on the genomic DNA extracted to amplify 16S rRNA gene and successfully amplified 16S rRNA gene from the samples was sent for sequencing.This research was approved by the IIUM Research Ethic Committee (IREC). Using the selected kit, genomic DNA was successfully extracted and PCR amplification of the bacterial 16S rRNAgeneusing universal bacterial primers was achieved giving a product of 1500 bp. Results from sequencing when analysedusing BLAST from the NCBI website, identified bacteria from the genus Vibrio, Haemophilus and Aggregatibacter from SECC samples while for caries-free samples Granulicatella was identified.

Item Type:	Article (Journal)
Additional Information:	4409/67343
Uncontrolled Keywords:	Severe Early Childhood Caries, polymerase chain reaction
Subjects:	R Medicine > RK Dentistry > RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry Kulliyyah of Dentistry > Department of Paediatric Dentistry and Dental Public Health Kulliyyah of Science Kulliyyah of Science > Department of Biotechnology
Depositing User:	Yunita Dewi Ardini
Date Deposited:	08 Nov 2018 15:31
Last Modified:	08 Nov 2018 15:31
URI:	http://irep.iium.edu.my/id/eprint/67343

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