Parametric study of soxhlet extraction of phenolic compounds from vernonia amygdalina leaves

Alara, Oluwaseun Ruth and Abdurahman, Nour Hamid and Abdul Mudalip, Siti Kholijah and Ukaegbu, Chinonso Ishmael and Azhari, Nour Hamid and Kabbashi, Nassereldeen Ahmed (2018) Parametric study of soxhlet extraction of phenolic compounds from vernonia amygdalina leaves. In: National Conference for Postgraduate Research 2018 (NCONPGR 2018), 28th-29th Aug. 2018, Kuantan, Pahang.

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Abstract

—Phenolic compounds are important secondary metabolites with different pharmacological activities. The effects of extraction parameters of Soxhlet extraction method which include extraction time, solid-to-solvent ratio and ethanol concentration on the recoveries of extraction yields, total phenolic content (TPC) and total flavonoid content (TFC) from extracts of Vernonia amygdalina leaves were investigated in this study using one-factor-at-a-time experiment (OFAT). Then,the functional group characterization of the extracts at maximum conditions was carried out. The results obtained showed that the three considered extraction parameters significantly influence the recoveries of extraction yield, TPC and TFC. Highest recoveries of extraction yield (14.22±0.19% w/w), TPC (75.94±2.15 mg GAE/g d.w.) and TFC (46.53±1.14 mg QE/g d.w.) were obtained at extraction time of 2 h, solid-to-solvent ratio of 1:20 g/mL and ethanol concentration of 60% v/v. Moreover, six major peaks were identified from the Soxhlet extraction of V. amygdalina leaves at maximum conditions using Fourier transform infrared spectroscopy (FTIR) analysis. Thus, this study showed that V. amygdalina leaf is a potential source of phenolic compounds which can be influence by the extraction parameters.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	4286/67165
Uncontrolled Keywords:	—Vernonia amygdalina; Soxhlet extraction; Total phenolic content; Total flavonoid content
Subjects:	T Technology > TP Chemical technology > TP155 Chemical engineering
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering Kulliyyah of Engineering
Depositing User:	Dr Nassereldeen Kabbashi
Date Deposited:	31 Oct 2018 11:40
Last Modified:	31 Oct 2018 11:40
URI:	http://irep.iium.edu.my/id/eprint/67165

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