A comparative study of the effects of different low-level lasers on the proliferation, viability, and migration of human melanocytes in vitro.

M. AlGhamdi, Khalid and Kumar, Ashok and Ashour, Abdelkader Elbadawy Abbas and A. AlGhamdi, Attieh (2015) A comparative study of the effects of different low-level lasers on the proliferation, viability, and migration of human melanocytes in vitro. Lasers in Medical Science, 30 (5). pp. 1541-1551. ISSN 0268-8921 E-ISSN 1435-604X

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Abstract

The aim of this study was to investigate the effects of different low-level laser therapies (LLLTs) of various wavelengths and energies on normal cultured human melanocytes. Various studies have shown the effects of LLLs on various types of cultured cells. Presently, little is known about the biological effects of LLLTs on melanocytes. Melanocytes were exposed to LLLT at 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, or 5.0 J/cm(2) using a blue (457 nm), red (635 nm), or ultraviolet (UV) (355 nm) laser. Melanocyte viability, proliferation, and migration were monitored at 72 h after irradiation. The blue (P < 0.001) and red (P < 0.001 and P < 0.01) lasers significantly enhanced viability at 0.5 to 2.0 J/cm(2), whereas the UV laser (P < 0.001) could significantly enhance viability only at 0.5 and 1.0 J/cm(2) compared with controls. The blue and red lasers also significantly enhanced the proliferation of the melanocytes at 0.5 to 2.0 J/cm(2) (P < 0.001), and the UV laser significantly enhanced proliferation at 0.5 to 1.5 J/cm(2) (P < 0.001 and P < 0.01) compared with controls. The blue laser significantly enhanced melanocyte migration at 0.5 to 4.0 J/cm(2) (P < 0.001 to P < 0.05), but the red (P < 0.001 and P < 0.01) and UV (P < 0.001 to P < 0.05) lasers could significantly enhance such migration at 0.5 to 1.0 J/cm(2) and 0.5 to 2.0 J/cm(2), respectively, compared with controls. LLLT at low energy densities is able to significantly increase melanocyte viability, proliferation, and migration in vitro, and at higher energy densities, it gives non-stimulatory results. Additionally, the blue laser was the best among the three lasers. These findings might have potential application in vitiligo treatment in future.

Item Type:	Article (Journal)
Additional Information:	66889/8129
Uncontrolled Keywords:	Low-level laser therapy, Cell culture, Viability, Proliferation, Melanocytes, Tissue engineering, Regenerative medicine
Subjects:	R Medicine > RL Dermatology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine > Department of Basic Medical
Depositing User:	Dr. Abdelkader Ashour
Date Deposited:	04 Jan 2019 16:04
Last Modified:	04 Jan 2019 16:04
URI:	http://irep.iium.edu.my/id/eprint/66889

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