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ATR-FTIR spectroscopic methods for analysis of black seed oil from alginate beads

Alkhatib, Hamzeh and Mohamed, Farahidah and Doolaanea, Abd Almonem (2018) ATR-FTIR spectroscopic methods for analysis of black seed oil from alginate beads. International Journal of Applied Pharmaceutics, 10 (5 (Sept.-Oct.)). pp. 147-152. ISSN 0975-7058

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Abstract

Objective: This study aimed to use attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR) for rapid quantification of black seed oil (BSO) from alginate beads and to develop simple analysis method for in vitro release study based on turbidity measurement. Methods: Guluronic acid-rich (high-G) sodium alginate was used to encapsulate BSO with the aid of Tween 80 as a stabilizer. ATR-FTIR method was developed for quantification of encapsulation efficiency of BSO by applying Beer-Lambert law after selection of a few wave number combinations. UV-vis method based on measurement of emulsion turbidity at 600 nm was also developed to quantify BSO during the releasing from alginate beads in simulated intestine buffer. Results: ATR-FTIR method exhibited linearity in the range of 25-300 mgBSO/mlemulsion (mgBSO/mlE)with R2=0.998, RSD=8.4%, LOD=0.28 mgBSO/mlE and LOQ=0.87 mgBSO/mlE. BSO-alginate beads was found to completely encapsulate BSO with around 100.5% efficiency. UV-vis method exhibited linearity in the range of 50-300 mgBSO/mlE, R2=0.9931, RSD=1.34%, LOD=0.89 mgBSO/mlE, and LOQ=2.71 mgBSO/mlE. In addition, the method showed that total amount of BSO was released at 110 min. Conclusion: These methods are considered as a practical method for quantification of BSO for encapsulation efficacy and release. They will help to accelerate and improve routine characterization of encapsulated BSO in food and pharmaceutical technology.

Item Type: Article (Journal)
Additional Information: 4341/66788
Uncontrolled Keywords: Black seed oil, Nigella sativa, ATR-FTIR, Encapsulation, Alginate, turbidity
Subjects: R Medicine > RS Pharmacy and materia medica
R Medicine > RS Pharmacy and materia medica > RS192 Materia Medica-Pharmaceutical Technology
R Medicine > RS Pharmacy and materia medica > RS403 Materia Medica-Pharmaceutical Chemistry
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Pharmacy
Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User: Assoc Prof Farahidah Mohamed
Date Deposited: 19 Oct 2018 15:46
Last Modified: 19 Oct 2018 15:46
URI: http://irep.iium.edu.my/id/eprint/66788

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