Optimization of citrus suhuiensis suspension culture conditions using Response Surface Methodology (RSM)

M. Fathil, Nur Alia and Mohamad Puad, Noor Illi and Azmi, Azlin Suhaida and Amid, Azura (2018) Optimization of citrus suhuiensis suspension culture conditions using Response Surface Methodology (RSM). In: 5th International Conference on Biotechnology Engineering (ICBioE 2018), 19th-20th September 2018, Kuala Lumpur.

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Abstract

Friable callus of Citrus suhuiensis (C. suhuiensis) derived from its cotyledon seed was inoculated for cell suspension culture establishment. Face Centred Central Composite Design (FCCCD) and Response Surface Methodology (RSM) in Design Expert 9.0 software were used to optimize C. suhuiensis cell suspension culture conditions. Prior to that, one-factor-at-a-time (OFAT) method was carried out to determine the suitable ranges of three factors for FCCCD which are carbon source, plant growth regulators (PGRs) and inoculum size. The cells were cultivated in Murashige and Skoog (MS) media supplemented with different concentrations of sucrose (40-60 g/L), 0.25-0.75 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 5-15% (v/v) inoculum. Result showed that the maximum cell dry weight was 21.42 g/L when media containing sucrose at 50 g/L, 2,4-D at 0.5 mg/L and 15% of inoculum were used. Based on ANOVA analysis, the model was significant while the p-value showed that the linear terms of 2,4-D and inoculum size as well as the quadratic terms of glucose and 2,4-D were highly significant (p<0.01). Linear and quadratic effects of 2,4-D were significant, indicating that small changes of 2,4-D would alter C. suhuiensis cell density.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	5462/66551
Uncontrolled Keywords:	Central Composite Design, C. suhuiensis, Response Surface Methodology, Carbon sources, PGR, Inoculum size
Subjects:	S Agriculture > SB Plant culture T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Noor Illi Mohamad Puad
Date Deposited:	12 Oct 2018 11:38
Last Modified:	12 Oct 2018 11:38
URI:	http://irep.iium.edu.my/id/eprint/66551

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