Comparison of bacterial loads from the lift buttons of a kulliyyah and a library at IIUM Kuantan and identification of the bacteria from selected buildings

Mohd Danial, Nur Izzatul Husna and Alias, Norsyuhada and Mohd Fauzi, Fadhin Sakina (2018) Comparison of bacterial loads from the lift buttons of a kulliyyah and a library at IIUM Kuantan and identification of the bacteria from selected buildings. In: Medical Research Symposium 2018, 29th Aug. 2018, Kuantan, Pahang. (Unpublished)

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Abstract

Introduction: Lift buttons are among the potential fomites in transmitting infectious microorganism to the community. The daily use of lift buttons by different people with various hygiene practices can contribute to the colonization of microorganism on the touched surfaces. There is few published research on microbial colonization on lift buttons in Malaysia, especially in the campus setting. Hence, this study aimed to identify and compare the bacterial load on lift buttons from a kulliyyah (faculty) and the library at IIUM Kuantan. Materials and Method: A total of 42 swab samples were collected aseptically from the lift buttons and cultured on nutrient, mannitol salt and MacConkey agar for bacterial enumeration and isolation. Gram staining, microscopy examination and molecular amplification of the 16S rDNA gene by polymerase chain reaction were performed for further characterization of the bacteria. Results: Out of 42 swab samples collected (kulliyyah: n =25, library: n=17), 88.4% of the samples confirmed the presence of bacteria with the lift buttons in the kulliyyah showed a higher percentage of bacterial isolation (92%) compared to the library (84%). Gram-positive cocci clustered bacteria dominated the isolates followed by Gram-positive bacilli, Gram-positive cocci tetrads and Gram-negative coccobacilli. Based on culture growth, Gram-staining features and 16S rDNA gene sequencing, isolated bacteria were identified as Staphylococcus sp., Staphylococcus kloosii, Bacillus cereus, Kocuria palustris, Yersinia mollaretii, Bacillus sp., Micrococcus sp., and other Gram-positive cocci. Conclusion: The results revealed a high bacterial load on lift buttons which can be a source of bacterial transmission.

Item Type:	Conference or Workshop Item (Speech/Talk)
Additional Information:	7690/66099
Uncontrolled Keywords:	lift buttons, kulliyyah, IIUM library, buildings
Subjects:	Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User:	Norsyuhada Alias
Date Deposited:	01 Oct 2018 14:23
Last Modified:	01 Oct 2018 14:23
URI:	http://irep.iium.edu.my/id/eprint/66099

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