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Application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against Candida albicans

Mukhtar, Nor Izzah and Abllah, Zurainie and Shahdan, Intan Azura (2018) Application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against Candida albicans. In: International Conference of Analytical Sciences (SKAM31), 17th-19th August 2018, Kuantan, Pahang. (Unpublished)

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Abstract

Candida albicans is becoming a significant problem for oral candidiasis worldwide. In addition, the proliferation of antifungal-resistant C. albicans has become a major concern. This study was carried out to evaluate the effects of activated virgin coconut oil (AVCO) and the crude extract of virgin coconut oil (VCO) to search for a new antifungal agent for treatment of oral candidiasis. The viability of C. albicans cells was determined using live/dead bacterial viability kit. C. albicans cells were grown in YPD broth culture overnight. The fungus was treated with AVCO and VCO at concentration of minimum inhibitory concentration (MIC), 6.24 µg/ml. To evaluate the viability of C. albicans cells, SYTO 9 and propidium iodide (PI) staining were used in this study and observed using fluorescence microscopy. C. albicans cells treated with AVCO showed more dead cells compared to cells treated with VCO. The data indicated that exposure of C. albicans to AVCO was the most inhibitory to growth (P<0.01).

Item Type: Conference or Workshop Item (Slide Presentation)
Additional Information: 5620/65973
Uncontrolled Keywords: Live-dead staining; antifungal activity; candida albicans; coconut oil
Subjects: R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Dentistry
Kulliyyah of Allied Health Sciences
Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Kulliyyah of Dentistry > Paediatric Dentistry, Orthodontics & Dental Public Health
Kulliyyah of Science
Kulliyyah of Science > Department of Biotechnology
Depositing User: Dr zurainie abllah
Date Deposited: 27 Sep 2018 17:32
Last Modified: 27 Sep 2018 17:32
URI: http://irep.iium.edu.my/id/eprint/65973

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