Flavonoids from Tetracera indica Merr. inhibit adipocyte differentiation and exert glucose uptake activities in 3T3-L1 adipocyte cells

Ahmed, Qamar Uddin and Hasan, Mohd Mehmudul and Sarian, Murni Nazira and Mat So'ad, Siti Zaiton and Bakhtiar, M. Taher and Latip, Jalifah (2016) Flavonoids from Tetracera indica Merr. inhibit adipocyte differentiation and exert glucose uptake activities in 3T3-L1 adipocyte cells. In: Regional Conference On Science, Technology & Social Sciences 2016 (RCSTSS), 4th - 6th December 2016, Cameron Highlands, Pahang. (Unpublished)

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Abstract

Tetracera indica Merr.(Family: DILLENIACEAE) is a large, woody, rain forest climber of Malaysia which is commonly known as Mempelas paya or sand paper plant. Two flavonoids MHQ-1 (wogonin) and MHQ-2 (norwogonin) were isolated from the stems ethanol extract of T. indica. Moreover, MHQ-1 and MHQ-2 including rosiglitazone as positive control were subjected to fluorescence glucose uptake test by 2-NBDG (fluorescent glucose analog) on mature adipocytes. Wogonin was found to show more cytotoxicity (IC50 <25 µg/mL) in comparison to norwogonin (no IC50 upto100 µg/mL) in MTT viability assay on 3T3-L1 preadipocyte cells. Structure activity relationships (SAR) study of both the flavonoids revealed that the methoxy (O-Me) substitution (wogonin) at position 8 of ring A of flavone skeleton is essential for inducing cytotoxicity on 3T3-L1 Pre-adipocytes. However, hydroxyl (OH) group at the same position (norwogonin) is safe to the cells (percent viability is above 80% for all the doses evaluated). Additionally, both the compounds were introduced to the cells in different safe concentrations as well as in different adipogenic cocktails. The adipogenic cocktails were modified by the compounds and rosiglitazone in the presence or absence of insulin. The results of adipogenesis showed that MHQ-1 and MHQ-2 induced adipogenesis like insulin and enhanced adipogenesis like rosiglitazone significantly in three different concentrations. Furthermore, both the compound stimulated the 2-NBDG uptake on mature adipocytes significantly. Results further confirm the traditional use of T. indica in the management of diabetes in Malaysia.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	4921/65520
Uncontrolled Keywords:	Tetracera indica Merr., dilleniaceae, flavonoids, 3T3-L1 preadipocyte cells, adipogenesis, glucose uptake activity
Subjects:	R Medicine > RS Pharmacy and materia medica > RS403 Materia Medica-Pharmaceutical Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User:	Dr. Siti Zaiton Mat So'ad
Date Deposited:	18 Sep 2018 12:30
Last Modified:	18 Sep 2018 12:30
URI:	http://irep.iium.edu.my/id/eprint/65520

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