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A simple method for extracting both active oily and water soluble extract (WSE) from Nigella sativa (L.) seeds using a single solvent system

Babar, Z.M. and Wan Sulaiman, Wan Mohd. Azizi and Arief Ichwan, Solachuddin Jauhari and Ahmed, Qamar Uddin and Azad, Abul Kalam and Mawa, Imranul (2018) A simple method for extracting both active oily and water soluble extract (WSE) from Nigella sativa (L.) seeds using a single solvent system. Natural Product Research. pp. 1-6. ISSN 1478-6419 E-ISSN 1478-6427 (In Press)

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Abstract

The current study provides a way of extraction for both active NSO and WSE from Nigella sativa seeds using 98% methanol. About 1 kg of ground seeds was macerated by 1:2.5 w/v (g/mL) for 72 hours. After rotary evaporation and 7 days of continuous drying and chilling at 50 and 4 °C, NSO and WSE were obtained at the same instant. Solubility tests of 24 solvents and 11 thin layer chromatographic analyses while 2, 2-diphenyl-1-picrylhydrazyl free radical scavenging assay of NSO (73.66) , WSE (33.32) and NSO + WSE (78.22) against ascorbic acid (IC50 = 4.28 mg/mL) was performed. WSE was found to be highly soluble in water and 5% NaOH exhibiting the same Rf value of 0.95 for EtOH:DMSO (9:1) against the honey. WSE has revealed more than twofold higher anti-oxidant activity than others. Formulation of WSE with Tualang honey may provide better targeted hydrophilic drug delivery systems.

Item Type: Article (Journal)
Additional Information: 4219/65108
Uncontrolled Keywords: N. sativa seeds, extraction, solubility, thin layer chromatography, antioxidant, NSO, WSE
Subjects: Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Dentistry
Kulliyyah of Pharmacy
Kulliyyah of Pharmacy > Department of Basic Medical Sciences
Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User: Dr Qamar Uddin Ahmed
Date Deposited: 15 Aug 2018 15:21
Last Modified: 15 Aug 2018 15:21
URI: http://irep.iium.edu.my/id/eprint/65108

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