Quantitative protein expression of Malaysian Phaleriamacrocarpa

Muhamad Bunnori, Noraslinda and Hamdan, Nurul Ashima and Abu Hassan, Muhamad Shafiq and Mohamed Rehan, Aisyah and Abd Halim, Khairul Bariyyah and Haron, Normah (2018) Quantitative protein expression of Malaysian Phaleriamacrocarpa. International Journal of Advances in Science Engineering and Technology , I, 6 (2). pp. 18-22. ISSN 2321-8991 E-ISSN 2321-9009

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Abstract

Herbal plants are the best alternative for synthetic medicines which are costly and proved to be double-edged source. P. macrocarpais the traditional herb compatible in curing diseases like diabetes, inflammation, and even cancer. People have gained lots of benefits from most parts of the plant with largest usage of seeds. This study aimed to analyze protein expressions in three major parts of the plant; leaf, seed and fruit by using SDS PAGE and high-throughput two-dimensional gel electrophoresis as proteomics approached. There were 42 spots detected in a pH range of 3-10 with 3 spots have been analyzed by MALDI-TOF mass spectrometry. Most identified proteins were expressed in seeds with highest intensity of bands and spots. The identified spots are homologs to putative clathrin assembly protein and ferredoxin NADP+ reductase which are characterized as ‘housekeeping agent’ in plant system.

Item Type:	Article (Journal)
Additional Information:	6604/64629
Uncontrolled Keywords:	Protein expression, phaleriamacrocarpa, 2D-PAGE, clathrin, ferredoxins
Subjects:	Q Science > QK Botany
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology Kulliyyah of Science
Depositing User:	Dr. Noraslinda Muhamad Bunnori
Date Deposited:	11 Jul 2018 16:00
Last Modified:	11 Jul 2018 16:00
URI:	http://irep.iium.edu.my/id/eprint/64629

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