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Treatment of osteomyelitis in New Zealand white rabbit using impregnated biomaterials with gentamicin: in vivo study outcome of bacterial study and micro-computed topography evaluation

Zulkifly, Ahmad Hafiz and Mohd Jan, Nurul Hafiza and Ibrahim, Mohd Zulfadzli and Aziz, Aziah (2017) Treatment of osteomyelitis in New Zealand white rabbit using impregnated biomaterials with gentamicin: in vivo study outcome of bacterial study and micro-computed topography evaluation. Malaysian Orthopaedic Journal, 11 (Supplement A). PR01C-PR01C. ISSN 1985-2533 E-ISSN 2232-111X

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INTRODUCTION: Treatment of osteomyelitis is still a major challenge in orthopaedic field. New Zealand White rabbit is acceptable experimental model described to develop effective local delivery of antibiotics osteomyelitis treatment as it can mimics the disease process in human. The objective of the study is to evaluate the outcome treatment of osteomyelitis in rabbit femur with impregnated Hydroxyappatite Gentamicin. Evaluation at stipulated durations using microCT Skyscan 1176 system. MATERIALS & METHODS: Osteomyelitis is created by inoculation of Staphylococcus aureus ATCC 25923 into rabbit’s distal femur. The rabbit were divided with 3, 6, 12, 26 week assessments. The response of the biomaterials (hydroxyapatite) impregnated with gentamicin against the infection were evaluated through microcomputed topography using Skyscan 1176 system.The bone analysis data were obtained by the software provided by Skyscan 1176 system. The distal femoral shaft were scanned using A1 1mm filter with 18 µm pixel resolutions. Bacterial study of culture and sensitivity, coagulase test, catalase test and gram stain were done to determine the treatment is effective. RESULTS: The data of bone analysis were for tissue volume (TV), bone surface (BS), bone volume (BV), and trabecular thickness (Tb.Th) in all groups were illustrated at Table 1. The bone healing noted in the treated group. The results showed no bacterial were grow in the agar and all test were negative at 26 weeks interval. Table 1 showing the bone analysis through Skyscan1176 system. DISCUSSIONS: Osteomyelitic changes were noted in all rabbit after inoculation of bacteria at week three and six. The micro-computed topographic analysis at 3, 6, 12 and 26 weeks of interval, showed increase bone parameters in treated femur. The bone volume (BV) was increased with value 124.96 mm3 compared to sham group (no treatment given) with bone volume 66.96 mm3. The density of biomaterials was decreased with duration of studies. This data showed that micro-computed topography studies is capable to provide information of bone microstructures in rapid and non-destructive way. The findings showed that impregnated biomaterials with gentamicin have potential to be used in osteomyelitis infection. CONCLUSION: Computed topography results showed at 26 weeks of treatment, the impregnated biomaterials have a potential in treatment of osteomyelitis.

Item Type: Article (Meeting Abstract)
Additional Information: 2397/64068
Uncontrolled Keywords: Micro-Computed Topography, Osteomyelitis
Subjects: R Medicine > RD Surgery > RD701 Orthopedics
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Medicine > Department of Department of Orthopaedics, Traumatology & Rehabilitation
Kulliyyah of Medicine
Depositing User: Mr. Mohd Zulfadzli Ibrahim
Date Deposited: 23 Jul 2018 10:43
Last Modified: 23 Jul 2018 10:43
URI: http://irep.iium.edu.my/id/eprint/64068

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