The determination of Acanthamoeba spp. from air ventilation system samples from selected buildings in Kuantan

Md Isa, Muhammad Lokman and Aznan, Anis Amira and Mat Amin, Nakisah and Mat Yusof, Afzan (2018) The determination of Acanthamoeba spp. from air ventilation system samples from selected buildings in Kuantan. International Journal of Care Scholars, 1 (1). pp. 19-21. E-ISSN 2600-898X

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Abstract

Objective: The present study is designed to investigate the occurrence of Acanthamoeba in air ventilation and air-conditioning systems in selected buildings in Kuantan. Methods: Acanthamoeba was isolated from dust samples taken from filters of air ventilation systems in selected buildings in Kuantan. The dusts were collected by using sterile cotton swabs, cultured in a xenic culture medium on non-nutrient agar (NNA) plates and incubated at 37º C. The plates were examined daily for any presence of Acanthamoeba cysts up to 7 days of incubation. Results: Based on the results obtained, there were no cysts or trophozoites of Acanthamoeba successfully isolated from all 75 dust samples. After three days of incubation, all culture plates examined show negative findings. Conclusions: The negative findings of this study were probably due to the limitations of the sampling method. It is recommended that future studies use the method proposed by National Institute of Occupational Safety and Health (NIOSH) for indoor air quality monitoring.

Item Type:	Article (Journal)
Additional Information:	5041/63303
Uncontrolled Keywords:	Acanthamoeba, air, ventilation systems, dust samples, immunosuppressed.
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Nursing > Department of Basic Medical Sciences for Nursing
Depositing User:	Dr. Afzan Mat Yusof
Date Deposited:	17 Apr 2018 10:21
Last Modified:	17 Apr 2018 10:21
URI:	http://irep.iium.edu.my/id/eprint/63303

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