Phytochemical constituents, a-glucosidase inhibitory activity and preliminary acute toxicity studies of the ethanol-water extracts of Salacca zalacca fruits in rats

Saleh, Mohammed S M and Siddiqui, Mohammad Jamshed Ahmad and Khatib, Alfi and Mat So'ad, Siti Zaiton (2018) Phytochemical constituents, a-glucosidase inhibitory activity and preliminary acute toxicity studies of the ethanol-water extracts of Salacca zalacca fruits in rats. In: 1st International Symposium on Herbal Medicine, Pre-conference of 3rd AMDI International Biohealth Sciences Conference (IBSC) 2018, 18th Jan. 2018, Kuching, Sarawak. (Unpublished)

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Abstract

Purpose: To evaluate the phytochemical constituent screening, α-glucosidase inhibitory activity and in vivo preliminary toxicity study of ethanol and water S. zalacca fruit extracts. Methods: The fresh fruit was collected, cleaned, peeled, and dried using freeze dryer. The flesh powder was extracted using solvent with different concentration of ethanol and water (100, 80, 60, 40, 20, 0%). All extracts were tested for α-glucosidase inhibitory activity and phytochemical constituents were evaluated using different chemical methods. The extract showing highest α-glucosidase inhibitory activity was tested for acute toxicity studies according to the up and down method. A dose (0.15, 0.48, 1.54, 4.91 and 5.00 g/ kg body weight) of the extract was used in 15 healthy male rats. The rats were observed for 4 h first day, then daily for 14 days. Result(s): The highest yield was determined for the extract of 80% concentration, whereas the highest α-glucosidase inhibitory activity (IC50 13.43±2.43 µg/mL), total phenolic content (TPC) (14.12+1.19 µg AAE/g) resulted for the extract of 60% concentration. The phytochemical screening showed the presence of saponins, carbohydrates and cardiac glycoside while alkaloids and flavonoids were not detected in all the extract. The five doses caused neither visible signs of toxicity nor mortality. The LD50 is above 5000 mg/kg. Conclusion(s): The ethanol and water extract possessed some active principles having -glucosidase inhibitory activity and is safe following single oral administration dose but not via the intraperitoneal route. Purpose: To evaluate the phytochemical constituent screening, α-glucosidase inhibitory activity and in vivo preliminary toxicity study of ethanol and water S. zalacca fruit extracts. Methods: The fresh fruit was collected, cleaned, peeled, and dried using freeze dryer. The flesh powder was extracted using solvent with different concentration of ethanol and water (100, 80, 60, 40, 20, 0%). All extracts were tested for α-glucosidase inhibitory activity and phytochemical constituents were evaluated using different chemical methods. The extract showing highest α-glucosidase inhibitory activity was tested for acute toxicity studies according to the up and down method. A dose (0.15, 0.48, 1.54, 4.91 and 5.00 g/ kg body weight) of the extract was used in 15 healthy male rats. The rats were observed for 4 h first day, then daily for 14 days. Result(s): The highest yield was determined for the extract of 80% concentration, whereas the highest α-glucosidase inhibitory activity (IC50 13.43±2.43 µg/mL), total phenolic content (TPC) (14.12+1.19 µg AAE/g) resulted for the extract of 60% concentration. The phytochemical screening showed the presence of saponins, carbohydrates and cardiac glycoside while alkaloids and flavonoids were not detected in all the extract. The five doses caused neither visible signs of toxicity nor mortality. The LD50 is above 5000 mg/kg. Conclusion(s): The ethanol and water extract possessed some active principles having -glucosidase inhibitory activity and is safe following single oral administration dose but not via the intraperitoneal route.

Item Type:	Conference or Workshop Item (Slide Presentation)
Additional Information:	6804/62481
Uncontrolled Keywords:	Phytochemical constituents, a-glucosidase inhibitory activity, Salacca zalacca fruits, rats
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr M Jamshed Siddiqui
Date Deposited:	17 Apr 2018 10:03
Last Modified:	17 Apr 2018 10:03
URI:	http://irep.iium.edu.my/id/eprint/62481

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