Extraction of lycopene from tomato waste using solid state fermentation

Jamal, Parveen and Hashlamona, Ali and Jaswir, Irwandi and Akbar, Iqrah and Wan Nawawi, Wan Mohd Fazli (2017) Extraction of lycopene from tomato waste using solid state fermentation. International Food Research Journal, 24 (Suppl). pp. 416-421. ISSN 1985-4668 E-ISSN 2231-7546

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Abstract

Lycopene is a well-known carotenoid, causing red color of fresh tomatoes. The significance of lycopene as antioxidant agent and coloring in the cosmetics, and its use in pharmaceutical and food industries has expanded in the recent years. Extraction of lycopene was improved effectively under solid state fermentation process; whereby, cellulase produced from the fermentation process was employed to degrade the cell-wall constituents, which facilitated the release of intracellular contents. The optimum conditions for the fermentation process were determined using Response Surface Methodology (RSM). The Facecentered Central Composite Design (FCCD) was employed to investigate the effects of three independent factors: moisture content in the range of 60 to 80 %, inoculum size ranging between 5 to 15% while the incubation time was set at 2, 3 and 4 days. Twenty runs of experiment were conducted and each one was repeated three times. The obtained data was analyzed using the Design Expert software v.6.0.8. Regression analysis showed that 94.56% of the variation was explained by the software. Under the optimized conditions, the highest lycopene yield was 307.2 µg/g when the moisture content was 80%, the inoculum size was 15% in 4 incubation days. The experimental values agreed with the predicted values, thus proving stability of the model used and the success of RSM. This study showed as to how fermentation can improve the extraction process by comparing the result with the control (extraction without fermentation) which was 0.8 µg/g.

Item Type:	Article (Journal)
Additional Information:	2937/62447
Uncontrolled Keywords:	Antioxidant, Carotenoid, Optimization, Fermentation, Lycopene
Subjects:	T Technology > T Technology (General) T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof.Dr. Parveen Jamal
Date Deposited:	02 Mar 2018 15:34
Last Modified:	25 Apr 2018 15:28
URI:	http://irep.iium.edu.my/id/eprint/62447

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