Comparative assessment of plasmid DNA delivery by encapsulation within or adsorbed on poly (D, L-lactide-co-glycolide) nanoparticles

Doolaanea, Abd Almonem and Mansor, Nur 'Izzati and Mohd Nor, Nurul Hafizah and Mohd Shafri, Mohd Affendi and Mohamed, Farahidah (2018) Comparative assessment of plasmid DNA delivery by encapsulation within or adsorbed on poly (D, L-lactide-co-glycolide) nanoparticles. Tropical Journal of Pharmaceutical Research, 17 (1). pp. 1-10. ISSN 1596-5996 E-ISSN 1596-9827

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Abstract

Purpose: To compare the gene delivery effectiveness of plasmid DNA (pDNA) encapsulated within poly (D,L-lactide-co-glycolide) (PLGA) nanoparticles with that adsorbed on PLGA nanoparticles. Methods: PLGA nanoparticles were prepared using solvent-evaporation method. To encapsulate pDNA within the particles, it was first complexed with cetyltrimethylammonium bromide (CTAB) and then added to the oil phase during the synthesis. For the adsorption, PLGA nanoparticles were first modified with either CTAB or chitosan and then pDNA was adsorbed on the particle surface by electrostatic interaction. Results: Nanoparticles encapsulating pDNA exhibited better plasmid loading and protection with significantly lower burst release (p < 0.05) compared to that of the nanoparticles with adsorbed plasmid. Cell uptake of chitosan-modified nanoparticles by murine neuroblastoma (N2a) cells was significantly (p < 0.05) higher than that of chitosan-free nanoparticles. Nanoparticles encapsulating pDNA showed higher transfection efficiency (p < 0.05) in N2a cells. Conclusion: Encapsulation of pDNA within PLGA nanoparticles presents a potential strategy for gene delivery that is superior to pDNA adsorbed on the nanoparticle surface. In addition, encapsulation keeps the particle surface free for further modifications such as the addition of targeting ligands.

Item Type:	Article (Journal)
Additional Information:	7245/62375
Uncontrolled Keywords:	Poly (D,L-lactide-co-glycolide), Plasmid DNA, Encapsulation, Adsorption, Cellular uptake, Gene therapy, Targeting ligands
Subjects:	R Medicine > RS Pharmacy and materia medica > RS192 Materia Medica-Pharmaceutical Technology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User:	Dr. Abd Almonem Doolaanea
Date Deposited:	14 Mar 2018 15:48
Last Modified:	24 Jan 2019 10:14
URI:	http://irep.iium.edu.my/id/eprint/62375

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