Isolation and screening of potential bacteria with biofilm formation ability and their hydrolytic enzymes for enhanced biogas production

Fazil, Nurul Alia and Alam, Md. Zahangir and Azmi, Azlin Suhaida and Mansor, Mariatul Fadzillah (2017) Isolation and screening of potential bacteria with biofilm formation ability and their hydrolytic enzymes for enhanced biogas production. In: International Conference on Environmental Research and Technology (ICERT 2017), 23rd-25th August 2017, Penang, Malaysia.

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Abstract

Biofilm mediated anaerobic digestion of POME are to be studied to produce biogas. Out of 120 strains isolated from palm oil mill effluent (POME), palm kernel cake (PKC), and food waste compost, only 13 strains showed biofilm producing ability with significant amount at OD595nm (>0.01). In hydrolytic enzyme assay test, which are amylolytic enzyme assay, cellulolytic enzyme assay, lipolytic enzyme assay, and proteolytic enzyme assay all strains does not secrete protease enzyme, and strain number 1C and 23 does not secrete any hydrolytic enzymes. Strain number 23C and 30C shows positive result for cellulase, amylase and lipase enzymes to be tested as single strain bacteria for prospect research on producing biogas while other strains will be treated as mixed culture in future research.

Item Type:	Conference or Workshop Item (Invited Papers)
Additional Information:	4157/61588
Uncontrolled Keywords:	Biofilm, hydrolytic enzyme, isolation, palm oil mill effluent
Subjects:	T Technology > TP Chemical technology > TP155 Chemical engineering
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr. Md. Zahangir Alam
Date Deposited:	26 Mar 2018 11:03
Last Modified:	24 Jan 2019 14:38
URI:	http://irep.iium.edu.my/id/eprint/61588

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