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Comparing impregnated of biomaterials with gentamicin as osteomyelitis treatment in New Zealand white rabbit – animal study

Zulkifly, Ahmad Hafiz and Mohd Jan, Nurul Hafiza and Aziz, Aziah and Ibrahim, Mohd Zulfadzli (2017) Comparing impregnated of biomaterials with gentamicin as osteomyelitis treatment in New Zealand white rabbit – animal study. In: Bio-CAM 2017 Conference on Biomedical & Advanced Material, 28th-29th November 2017, Langkawi, Kedah. (Unpublished)

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Treatment of osteomyelitis is still a major problem in orthopedic field. New Zealand White rabbit (NZWR) is acceptable experimental model described to develop effective local delivery of antibiotics osteomyelitis treatment as it can mimics the disease process in human. The objective of this study was to analyze the treatment given via impregnated biomaterials with gentamicin beads in rabbit models. Osteomyelitis was developed by inoculation of Staphylococcus aureus ATCC 25923 into the drilled rabbit’s distal femur. Thirty-two (32) NZWR was used during the research. The rabbits were divided into 2 groups; (hydroxyapatite (HA) and calcium sulphate (CaSO4)) with four subgroups assessments in (3, 6, 12 and 26) weeks. The responses of the treatments against infection were evaluated through gross presentations, radiograph, micro CT, microbiology and histology. The rabbits were sacrificed accordingly to evaluate the healing process of bone. Results showed osteomyelitis changes were noted in all rabbit after inoculation of bacteria at week 3 intervals. Intervention was done after 3 weeks of inoculation. Chronic osteomyelitis was confirmed via radiology and microbial assessments. SPSS Version 23 was used to evaluate the results. The microbiology analysis revealed there was no bacterium isolated at 26th week interval in both group. Histology interpretation, healing was noted at site with appearance of new bone formation at 6 to 26 weeks of intervals. The micro CT results showed that trabeculae numbers increased significantly (p<0.05) with the treatment. The results show bone healing at 26th week of interval. There was no significant difference between both treatments modalities in the area of radiology, histology and micro CT assessment (p<0.05). The impregnated antibiotics biomaterial have its function in the treatment of osteomyelitis. In conclusion, these result showed that impregnated biomaterials with gentamicin have roles in treatment of osteomyelitis as added to medical and surgical treatment.

Item Type: Conference or Workshop Item (Poster)
Additional Information: 2397/61457
Uncontrolled Keywords: New Zealand White Rabbit; Staphylococcus aureus; Impregnated Biomaterials; Osteomyelitis
Subjects: R Medicine > RD Surgery > RD701 Orthopedics
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Medicine > Department of Department of Orthopaedics, Traumatology & Rehabilitation
Depositing User: Mr. Mohd Zulfadzli Ibrahim
Date Deposited: 17 Jan 2018 15:59
Last Modified: 17 Jan 2018 15:59
URI: http://irep.iium.edu.my/id/eprint/61457

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