Cancer chemoprevention study of luffa aegyptiaca (sponge gourd) seed extract on human breast cancer cell lines (MCF-7)

Hassan, Habibah and Razali, Shakirah and Firus Khan, Al'aina Yuhainis and K Jalal, Tara and Salleh, Azil and Khatib, Alfi and Hashim, Ridzwan and Abdul Wahab, Ridhwan (2016) Cancer chemoprevention study of luffa aegyptiaca (sponge gourd) seed extract on human breast cancer cell lines (MCF-7). In: 3rd Pan Asian Biomedical Science Conference 2016, 7-8th December 2016, Management and Science University (MSU), in Shah Alam, Selangor Darul Ehsan, Malaysia. (Unpublished)

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Abstract

Natural products from plant have been used for centuries to prevent various diseases including cancer. Many chemopreventive agents are believed to inhibit or delay the progression of cancer cells through suppression of cell proliferation, induction of apoptosis and cell cycle arrest. In Malaysia, breast cancer is the most common form of cancer affecting women. The prognosis for breast cancer is still poor and conventional treatment is not always effective. Therefore, this study aims to determine the chemopreventive effect of Luffa aegyptiaca seed extract (LSE) on breast cancer through anti-proliferative effect and its mechanisms on breast cancer cell (MCF-7) as evidenced by the result of IC50 determination, cell proliferation assay and flow cytometer analysis on apoptosis induction and cell cycle arrest. MCF-7 cells were plated at a density of 4.7 x 104 cells/ml in 6–well plate. Trypan blue exclusion method was used to determine cell viability. Untreated and LSE-treated MCF-7 cells were observed for morphological changes with an inverted-phase contrast microscope. The induction of apoptosis and cell cycle arrest was analysed using flow cytometry. LSE showed growth inhibitory effect with IC50 value at 0.0625 mg/ml. Moreover, LSE, show no toxic effect on normal cells possibly showing that it is a potential chemopreventive agent on MCF-7 via the induction of apoptosis and cell cycle arrest. Overall, the data collected provides new insight for the use of Luffa aegyptiaca seed as a chmopreventive agent on MCF-7. Keywords: Luffa aegyptiaca; anti-proliferative effect; apoptosis; cell cycle; MCF-7

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	6915/61123
Uncontrolled Keywords:	Cancer chemoprevention; Luffa aegyptiaca (sponge gourd) seed extract; Human breast cancer; Cell lines (MCF-7)
Subjects:	S Agriculture > SF Animal culture
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User:	Dr Ridhwan Abdul Wahab
Date Deposited:	23 Jan 2018 14:56
Last Modified:	23 Jan 2018 14:56
URI:	http://irep.iium.edu.my/id/eprint/61123

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