Understanding of anti-cancer properties of Neolamarckia cadamba (Kalempayan) leaves extract on breast cancer cell

Razali, Shakirah and Hassan, Habibah and Firus Khan, Al'aina Yuhainis and K Jalal, Tara and Abdul Jamil, Muhammad Mahadi and Johan, Muhammad Farid and Mohammad, Mardhiah and Draman, Samsul and Salleh, Azir and Khatib, Alfi and Hashim, Ridzwan and Abdul Wahab, Ridhwan (2016) Understanding of anti-cancer properties of Neolamarckia cadamba (Kalempayan) leaves extract on breast cancer cell. In: 3rd Pan-Asian Biomedical Science Conference 2016 (3rd PABSC), 7th–8th December 2016, Shah Alam, Selangor. (Unpublished)

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Abstract

Natural products have been used in treating illnesses since ancient times. Neolamarckia cadamba (N. cadamba) plant, locally known as Kalempayan, is an Ayurvedic medicine. It has been used by Indian folklore for treatment such as fever, anaemia, stomatitis, and infectious diseases. This Rubiaceae family plant indicates that it has a therapeutic potential against diseases, including cancer. Therefore, this study aimed to investigate the chemopreventive effect of N. cadamba’s leaves extract (NCE) with regard to the induction of apoptosis and cell cycle arrest against human breast adenocarcinoma cancer cell-line (MCF-7). MCF-7 cells were seeded at 4.7 x 104 cells/well in 6-well plates. The cells were treated with series concentration of NCE. The 50% inhibitory concentration (IC50) of NCE and its antiproliferative effect were measured using Trypan blue exclusion method was determined as 0.125 mg/ml. The flow cytometric analysis was performed in MCF-7 revealed the NCE inhibited the cell proliferation through induction of apoptosis and cell cycle arrest. The present study demonstrates that the NCE has a positive potential as a chemopreventive agent. Further research need to be done to identify the molecular mechanism related to induction of apoptosis and cell cycle arrest in breast cancer cells.

Item Type:	Conference or Workshop Item (Speech/Talk)
Additional Information:	5448/61120
Uncontrolled Keywords:	Neolamarckia cadamba; apoptosis induction; cell cycle arrest; breast cancer
Subjects:	Q Science > QH Natural history > QH301 Biology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Medicine > Department of Family Medicine (Effective: 1st January 2011) Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr Ridhwan Abdul Wahab
Date Deposited:	22 Jan 2018 15:22
Last Modified:	22 Jan 2018 15:22
URI:	http://irep.iium.edu.my/id/eprint/61120

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