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Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach

Perumal, Vikneswari and Ahmed, Qamar Uddin and Khatib, Alfi and Uzir, Bisha Fathamah and Murugesu, Suganya (2017) Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach. In: 2nd International Anatomical and Biomedical Scientific Conference 2017, 1-2 Aug 2017, Serdang, Selangor.

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Objectives: The present study was design to identify the significant biomarkers from M.charantia that possessing antioxidant activity using liquid chromatography-mass spectrometry (LC-MS) based metabolomics approaches. Design and method: Initially, the fruit was extracted by soaking in different solvents with different concentrations of ethanol in water (0, 20, 40, 60, 80, 100%, v/v). Then, the extracts were tested for antioxidant activity using 1, 1-diphenyl-2 picrylhydrazyl (DPPH) and ferric reducing antioxidant power assay. LC-MS based metabolomics approach correlated with multivariate data analysis was applied to profile the bioactive compounds present in the extract. Results The 80% ethanol extract showed a high inhibitory activity on 1, 1-diphenyl-2 picrylhydrazyl (DPPH) radical and high ferric reducing antioxidant power, LC-MS based metabolomics approaches helped to identify several antioxidants in this extract such as ascorbic acid, margarolic acid, brevifolincarboxylic acid, quercetin 3-O-glycoside, kuguacin H, cucurbitacin E, 3-malonylmomordicin I, goyaglycoside G. Conclusion: The results of the present study shows possible antioxidants from M. charantia fruit can be identified Keywords: Momordica charantia, LCMS, Metabolomics, antioxidant, multivariate data analysis.

Item Type: Conference or Workshop Item (Speech/Talk)
Additional Information: 4921/61010
Subjects: Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User: Dr Qamar Uddin Ahmed
Date Deposited: 31 Dec 2017 22:37
Last Modified: 31 Dec 2017 22:41
URI: http://irep.iium.edu.my/id/eprint/61010

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