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In vitro evaluation of porcupine bezoar extracts as anticancer agent on A549 -A preliminary study

Firus Khan, Al'aina Yuhainis and Mohd Nasir, Mohd Hamzah and Jalal, Tara Kamal and Razali, Shakirah and Hassan, Habibah and Abdullah Asuhaimi, Faizah and Abdul Wahab, Ridhwan (2017) In vitro evaluation of porcupine bezoar extracts as anticancer agent on A549 -A preliminary study. Advances in Biotechnology and Microbiology, 5 (1). pp. 1-8. ISSN 2474-7637

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Porcupine bezoar (PB) was reported to possess medicinal properties in old medical manuscript. However, its potential as anticancer agent on human lung cancer cells (A549) is not yet studied. In present study, porcupine bezoar was tested to observe its ability in inhibiting cell growth of cancer cell (A549) and its cytotoxicity on Normal Human Gingival Fibroblast Cell (HGF-1). A549 cells morphology was observed after treated with bezoars for 72 hours. The ability of bezoars to induce DNA damage and apoptosis was analyzed by staining cells with Hoechst 33428(nucleus) and Rhodamine Phalloidin (f-actin). The A549 IC50 is 13.6±1.58μg/ml A549 growths was inhibited in dose-dependent pattern, but no inhibition found on normal HGF-1 cells. Treated A549 morphology shows sign of apoptosis such as DNA fragmentation, cytoplasm shrunk and vacuolation. The finding in this study suggests PB extracts able to inhibit cell growth, induce DNA damage and apoptosis, further analysis need to be done to verify the mechanism.

Item Type: Article (Journal)
Additional Information: 5522/60815
Uncontrolled Keywords: A549; Porcupine bezoar; Proliferation; Apoptosis; DNA damage
Subjects: R Medicine > RM Therapeutics. Pharmacology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences
Kulliyyah of Science
Depositing User: Dr Ridhwan Abdul Wahab
Date Deposited: 05 Jan 2018 11:16
Last Modified: 05 Jan 2018 11:16
URI: http://irep.iium.edu.my/id/eprint/60815

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