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Saliva leptin levels in tooth movement during initial stage of orthodontic alignment: a pilot study

Abu Bakar, Noraini and Kamil, Wisam Alaa and Al-Bayati, Lina Hilal Abbood and Mustafa Al-Ahmad, Basma Ezzat (2017) Saliva leptin levels in tooth movement during initial stage of orthodontic alignment: a pilot study. Brazilian Journal of Oral Sciences, 16. pp. 1-8. ISSN 1677-3225 E-ISSN 1677-3217

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INTRODUCTION During orthodontic tooth movement, the early response of periodontal tissues to mechanical stress is an acute inflammatory reaction. Mechanical stress from orthodontic appliances is believed to induce cells in the periodontal ligament (PDL) to form biologically active substances, such as enzymes and cytokines, responsible for connective tissue remodeling (Nishijima Y et al 2006). Leptin, a polypeptide hormone has been classified as a cytokine (Zhang et al 1994). Earlier findings concluded that leptin at high local concentrations protects the host from inflammation and infection as well as maintaining bone levels. It has been also suggested that leptin plays a significant role in bone formation by its direct effect on osteoblasts (Alparslan et al 2010). This pilot study aimed to study leptin in saliva and its association with tooth movement during initial orthodontic alignment. OBJECTIVES To determine if there are any differences in saliva leptin level before and after orthodontic alignment MATERIAL AND METHODS Ten orthodontic patients (7 girls and 3 boys; mean age, 16.76 ± 1.1 years) with crowding (up to 5mm) that required orthodontic fixed appliances, on a non-extraction basis as part of the treatment plan, were recruited in this longitudinal study. Orthodontic study models were constructed at baseline and at 6- weeks after orthodontic treatment commenced. Full fixed orthodontic appliances with initial 0.014” Nickel Titanium archwire placed. The amount of crowding was measured, before and after initial alignment with an electronic digital caliper (Max-Cal, Japan Micrometer Ltd, Tokyo, Japan) with an accuracy of up to 0.01mm. Unstimulated morning saliva sample were collected at all visits, after at least an 8-hour period of fasting and no-toothbrushing. After centrifugation (4000x g;10min), the samples were stored at -25C and tested using Leptin Abnova LEP Human ELISA kit (KA3080) which was subsequently analyzed. Subjects’ periodontal health status was also monitored throughout the study. Ethical approval (ID IREC 262) was received on 7th April 2014 from International Islamic University Malaysia Research Ethics Committee (IREC). RESULTS Leptin concentration in saliva was significantly decreased in a time-dependant manner (t(9)=8.60, p<0.001), from before orthodontic treatment (7016.45± 425.15 pg/mL) and 6 weeks after bond-up (4901.92± 238.64 pg/mL). CONCLUSION Leptin concentration in saliva is decreased during orthodontic tooth movement in initial alignment stage. KEYWORDS Leptin, saliva, tooth movement

Item Type: Article (Journal)
Additional Information: 5978/60191
Uncontrolled Keywords: Leptin, saliva, tooth movement
Subjects: R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Dentistry
Depositing User: Asst Prof Dr Noraini Abu Bakar
Date Deposited: 18 Dec 2017 18:12
Last Modified: 04 Mar 2018 17:18
URI: http://irep.iium.edu.my/id/eprint/60191

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