Comparative assessment of poly (D, L-lactide-co-glycolide) nanoparticles modified by either cetyltrimethylammonium bromide or chitosan for plasmid DNA adsorption

Doolaanea, Abd Almonem and Mansor, Nur 'Izzati and Nor, Nurul Hafizah Mohd and Mohd Shafri, Mohd Affendi and Susi, Sukmasari and Mohamed, Farahidah (2017) Comparative assessment of poly (D, L-lactide-co-glycolide) nanoparticles modified by either cetyltrimethylammonium bromide or chitosan for plasmid DNA adsorption. Tropical Journal of Pharmaceutical Research, 16 (10). pp. 2315-2324. ISSN 1596-5996 E-ISSN 1596-9827

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Abstract

Purpose: To evaluate poly (D,L-lactide-co-glycolide) PLGA nanoparticles modified by cetyltrimethyl ammonium bromide (CTAB) or chitosan for plasmid DNA adsorption. Methods: PLGA nanoparticles were prepared by solvent diffusion method and modified by including CTAB in the aqueous (F1) or oil phase (F2), or by including low (F3) or medium (F4) molecular weight chitosan. The nanoparticles were characterised by differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FTIR), as well as for cell toxicity, cell uptake and transfection. Results: CTAB failed to confer positive charge on the nanoparticles. CTAB desorbed easily from F1 surface. This resulted in negative zeta potential, increased cytotoxicity as well as decreased cell uptake and transfection. In F2, CTAB was located mainly in PLGA matrix, resulting in negative charge with decreased cytotoxicity, and increased cell uptake and transfection compared to F1. On the other hand, chitosan-modified nanoparticles (F3 and F4) showed stronger interaction between chitosan and PLGA, leading to positively-charged particles, decreased cytotoxicity, as well as increased cell uptake and transfection. Amongst the four formulations, F4 exhibited the highest transfection. Conclusion: These results should aid in understanding how PLGA nanoparticles are modified by CTAB and chitosan. Modification with chitosan yields PLGA nanoparticles with higher DNA adsorption and transfection with lower cytotoxicity.

Item Type:	Article (Journal)
Additional Information:	7245/60006
Uncontrolled Keywords:	Chitosan, cetyltrimethyl ammonium bromide (CTAB), Nanoparticle, Poly (D,L-lactide-coglycolide) PLGA, Plasmid DNA adsorption, Gene therapy
Subjects:	R Medicine > RS Pharmacy and materia medica > RS192 Materia Medica-Pharmaceutical Technology R Medicine > RS Pharmacy and materia medica > RS403 Materia Medica-Pharmaceutical Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User:	Dr. Abd Almonem Doolaanea
Date Deposited:	13 Dec 2017 17:58
Last Modified:	10 Jul 2018 17:34
URI:	http://irep.iium.edu.my/id/eprint/60006

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