Statistical optimization of gelatin immobilization on modified surface PCL microcarrier to improve PCL microcarrier compatibility

Samsudin, Nurhusna and Hashim, Yumi Zuhanis Has-Yun and Arifin, Mohd Azmir and Mel, Maizirwan and Mohd. Salleh, Hamzah and Nordin, Norshariza and Sopyan, Iis and Jimat, Dzun Noraini (2017) Statistical optimization of gelatin immobilization on modified surface PCL microcarrier to improve PCL microcarrier compatibility. Jurnal Teknologi, 79 (6). pp. 167-175. ISSN 2180-3722

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Abstract

Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent mechanical properties and degradation kinetics that suit various applications, but its non-polar hydrocarbon moiety renders it sub-optimal for cell attachment. In this present study, the aim was to improve biocompatibility of PCL microcarrier by introducing oxygen functional group via ultraviolet irradiation and ozone aeration (UV/O3 system) to allow covalent immobilization of gelatin on the PCL microcarrier surface. Respond surface methodology was used as a statistical approach to optimized parameters that effect the immobilization of gelatin. The parameters used to maximized amount of gelatin immobilize were the mol ratio of COOH:EDAC, NHS concentration and gelatin concentration. The optimum conditions for maximum amount of gelatin (1797.33 μg/g) on the surface of PCL were as follows: 1.5 of COOH:EDAC ratio, 10 mM NHS concentration and, 80 mg/ml gelatin. The result shows that gelatin coated PCL microcarrier promote more and rapid cell adhesion with density of 16.5 ×105 cells/ml as compared to raw PCL microcarrier (2.4×105cells/ml) and UV/O3 treated PCL microcarrier (4.25×105cells/ml). Therefore, immobilization of gelatin with optimized parameters onto PCL microcarrier improved biocompatibility of PCL microcarrier.

Item Type:	Article (Journal)
Additional Information:	4809/59803
Uncontrolled Keywords:	Microcarrier, gelatin immobilization, polycaprolactone, statistical optimization
Subjects:	T Technology > TA Engineering (General). Civil engineering (General) > TA164 Bioengineering
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART) Kulliyyah of Engineering > Department of Biotechnology Engineering Kulliyyah of Engineering > Department of Manufacturing and Materials Engineering
Depositing User:	PROF. DR. IIS SOPYAN
Date Deposited:	06 Dec 2017 14:53
Last Modified:	17 May 2018 15:20
URI:	http://irep.iium.edu.my/id/eprint/59803

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