Toxicity studies of natural product in vero cells using impedance monitoring

Hamdan, Taufik Hakim and Mansor, Ahmad Fairuzabadi and Abbas, Phirdaous and Nordin, Anis Nurashikin and Hashim, Yumi Zuhanis Has-Yun (2017) Toxicity studies of natural product in vero cells using impedance monitoring. In: International Medical Device and Technology Conference (iMEDiTEC 2017), 6th-7th September 2017, Johor Bahru, Johor.

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Abstract

Natural products have traditionally been used for medicinal purposes in Asian communities. Toxicity studies typically use animal testing to predict the harmfulness of a particular substance to human health. For this study, in lieu of animal testing, we utilize cell-based biosensors to evaluate the toxicity of natural products. The cell-based biosensors are fabricated on a printed circuit board with copper electrodes and are equipped with PDMS cell culture chambers. Two different electrodes (interdigitated and circular) were designed. Vero cells were used to represent normal healthy cells. The cells are first cultured on biosensors and then are inoculated with natural products, Taxol (chemo drug – positive control) and DMSO (negative control). Impedances of these biosensors were then recorded at six-hour intervals for 80 hours to determine the growth of the cells. It was found that compared to Taxol, natural products have a very low toxicant values.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	3774/59736
Uncontrolled Keywords:	Toxicity studies, natural product, vero cells
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering Kulliyyah of Engineering > Department of Biotechnology Engineering Kulliyyah of Engineering > Department of Electrical and Computer Engineering
Depositing User:	DR YUMI ZUHANIS HAS-YUN HASHIM
Date Deposited:	30 Nov 2017 16:02
Last Modified:	30 Nov 2017 16:02
URI:	http://irep.iium.edu.my/id/eprint/59736

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