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Surface modification of polycaprolactone (PCL) microcarrier for performance improvement of human skin fibroblast cell culture

Samsudin, Nurhusna and Hashim, Yumi Zuhanis Has-Yun and Arifin, Mohd Azmir and Mel, Maizirwan and Mohd Salleh, Hamzah and Sopyan, Iis (2018) Surface modification of polycaprolactone (PCL) microcarrier for performance improvement of human skin fibroblast cell culture. In: International Conference on Advances in Manufacturing and Materials Engineering 2017 (ICAMME17), 8th-9th August 2017, Kuala Lumpur.

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Abstract

Polycaprolactone (PCL) has many advantages for use in biomedical engineering field. In the present work PCL microcarriers of 150-200 µm were fabricated using oil-in-water (o/w) emulsification coupled with solvent evaporation method. The surface charge of PCL microcarrier was then been improved by using ultraviolet/ozone treatment to introduce oxygen functional group. Immobilisation of gelatin onto PCL microspheres using zero-length crosslinker provides a stable protein-support complex, with no diffusional barrier which is ideal for mass processing. The optimum concentration of carboxyl group (COOH) absorbed on the surface was 1495.9 nmol/g and the amount of gelatin immobilized was 1797.3 µg/g on UV/O3 treated microcarriers as compared to the untreated (320 µg/g) microcarriers. The absorption of functional oxygen groups on the surface and the immobilized gelatin was confirmed with the attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR) and the enhancement of hydrophilicity of the surface was confirmed using water contact angle measurement which decreased (86.93o – 49.34o) after UV/O3 treatment and subsequently after immobilisation of gelatin. The attachment and growth kinetics for human skin fibroblast cell (HSFC) showed that adhesion occurred much more rapidly for gelatin immobilised surface as compared to untreated PCL and UV/O3 PCL microcarrier.

Item Type: Conference or Workshop Item (Plenary Papers)
Additional Information: 3774/59734
Uncontrolled Keywords: surface modification, polycaprolactone (PCL) microcarrier, human skin fibroblast, cell culture
Subjects: Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: DR YUMI ZUHANIS HAS-YUN HASHIM
Date Deposited: 30 Nov 2017 15:23
Last Modified: 21 Mar 2018 10:46
URI: http://irep.iium.edu.my/id/eprint/59734

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