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Imbalanced expression of polycistronic miRNA in acute myeloid leukemia

Kotaki, Ryutaro and Higuchi, Hiroshi and Ogiya, Daisuke and Katahira, Yasuhiro and Kurosaki, Natsumi and Yukihira, Naoko and Ogata, Jun and Yamamoto, Haruna and Mohamad Alba, Syakira and Azhim, Azran and Kitajima, Tatsuo and Inoue, Shigeaki and Morishita, Kazuhiro and Ono, Koh and Koyama-Nasu, Ryo and Kotani, Ai (2017) Imbalanced expression of polycistronic miRNA in acute myeloid leukemia. International Journal of Hematology. pp. 1-9. ISSN 0925-5710 E-ISSN 1865-3774 (In Press)

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miR-1 and miR-133 are clustered on the same chromosomal loci and are transcribed together as a single transcript that is positively regulated by ecotropic virus integration site-1 (EVI1). Previously, we described how miR-133 has anti-tumorigenic potential through repression of EVI1 expression. It has also been reported that miR-1 is oncogenic in the case of acute myeloid leukemia (AML). Here, we show that expression of miR-1 and miR-133, which have distinct functions, is differentially regulated between AML cell lines. Interestingly, the expression of miR-1 and EVI1, which binds to the promoter of the miR-1/miR-133 cluster, is correlative. The expression levels of TDP-43, an RNA-binding protein that has been reported to increase the expression, but inhibits the activity, of miR-1, were not correlated with expression levels of miR-1 in AML cells. Taken together, our observations raise the possibility that the balance of polycistronic miRNAs is regulated post-transcriptionally in a hierarchical manner possibly involving EVI1,suggesting that the deregulation of this balance may play some role in AML cells with high EVI1 expression.

Item Type: Article (Journal)
Additional Information: 7380/58944
Uncontrolled Keywords: miR-1, miR-133, EVI1, AML
Subjects: R Medicine > RC Internal medicine > RC254 Neoplasms. Tumors. Oncology (including Cancer)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Science > Department of Biotechnology
Depositing User: Ir Dr Azran Azhim Noor Azmi
Date Deposited: 01 Nov 2017 10:11
Last Modified: 16 Jan 2018 12:14
URI: http://irep.iium.edu.my/id/eprint/58944

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