Biflavonoids from the leaves and stem bark of Garcinia griffithii and their biological activities

Wan Salleh, Wan Mohd Nuzul Hakimi and Nik Sazali, Nik Shazwani Afifah and Ahmad, Farediah and Bakhtiar, M. Taher (2017) Biflavonoids from the leaves and stem bark of Garcinia griffithii and their biological activities. Marmara Pharmaceutical Journal, 21 (4). pp. 889-897. ISSN 1309-0801

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Abstract

The phytochemical and biological activity of the leaves and stem barks of Garcinia griffithii have been investigated. Extraction and chromatographic purification of the leaves and stem barks extracts have successfully afforded five biflavonoids namely amento- 4’’-methylether (1), 3,8’’-binaringenin (2), morelloflavone (3), 3,8’’-binaringenin-7’’-O-glucoside (4) and morelloflavone-7’’-Oglucoside (5), together with squalene (6), canophyllol (7), friedelin (8) and β-amyrin (9) which were characterized spectroscopically. All extracts and phytochemicals were tested for antioxidant, antityrosinase and antibacterial activities. The antioxidant assay on DPPH radical scavenging showed that the n-hexane extract of the stem barks had the highest radical scavenging activity with IC50 value of 96.4 μg/mL, while compound (3) was found to be the strongest antioxidant compound with IC50 value of 57.6 μg/mL. The methanol extract of the leaves showed the highest total phenolic content with 444.1 mg/g of gallic acid equivalent (GAE/L) and 423.1 mg/g of cathechin equivalent (CE/L). The extracts and all compounds were found to have weak antityrosinase activity. The antimicrobial assays of all the extracts were carried out by minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC). The methanol extract of the leaves showed the most significant antimicrobial activity towards E. faecalis and K. pneumoniae with MIC and MBC value ranged between 225-450 μg/mL compared to the other extracts.

Item Type:	Article (Journal)
Additional Information:	5209/58842
Uncontrolled Keywords:	Garcinia griffithii ; antioxidant ; antityrosinase ; antibacteria
Subjects:	R Medicine > RS Pharmacy and materia medica R Medicine > RS Pharmacy and materia medica > RS403 Materia Medica-Pharmaceutical Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User:	Dr Muhammad Taher
Date Deposited:	24 Oct 2017 15:49
Last Modified:	05 Apr 2018 14:55
URI:	http://irep.iium.edu.my/id/eprint/58842

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