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p16 tumor suppressor gene methylation in diffuse large B cell lymphoma: a study of 88 cases at two hospitals in the East Coast of Malaysia

Mohd. Ridah, Lailatul Jalilah and A. Talib, Norlelawati and Muhammad, Siti Aesah @ Naznin and Hussain, Faezahtul Arbaeyah and Zainuddin, Norafiza (2017) p16 tumor suppressor gene methylation in diffuse large B cell lymphoma: a study of 88 cases at two hospitals in the East Coast of Malaysia. Asian Pacific Journal of Cancer Prevention, 18 (10). pp. 2781-2785. ISSN 1513-7368 E-ISSN 2476-762X (In Press)

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Abstract

Introduction: p16 gene plays an important role in the normal cell cycle regulation. Methylation of p16 has been reported to be one of the epigenetic events contributing to the pathogenesis of diffuse large B-cell lymphoma (DLBCL) which occurring at varying frequency. DLBCL is an aggressive and high-grade malignancy which accounts for approximately 30% of all non-Hodgkin lymphoma cases. However, little is known regarding the epigenetic alterations of p16 gene in DLBCL cases in Malaysia. Therefore, the objective of this study was to examine the status of p16 methylation in DLBCL. Methods: A total of 88 formalin-fixed paraffin-embedded DLBCL tissues retrieved from two hospitals located in the east coast of Malaysia, namely Hospital Tengku Ampuan Afzan (HTAA) Pahang and Hospital Universiti Sains Malaysia (HUSM) Kelantan, were chosen for this study. DNA specimens were isolated and subsequently subjected to bisulfite treatment prior to methylation specific-PCR. Two pairs of primers were used to amplify methylated and unmethylated regions of p16 gene. The PCR products were then separated using agarose gel electrophoresis and visualised under UV illumination. SPSS version 12.0 was utilised to perform all statistical analysis. Result: p16 methylation was detected in 65 of 88 (74%) samples. There was a significant association between p16 methylation status and patients aged >50 years old (p=0.04). Conclusion: Our study demonstrated that methylation of p16 tumor suppressor gene in our DLBCL cases is common and significantly increased among patients aged 50 years and above. Aging is known to be an important risk factor in the development of cancers and we speculate that this might be due to the increased transformation of malignant cells in aging cell population. However, this has yet to be confirmed with further research and correlate the findings with clinicopathological parameters.

Item Type: Article (Journal)
Additional Information: 4667/58746
Uncontrolled Keywords: DNA methylation; methylation specific PCR; p16; diffuse large B cell lymphoma; Malaysia
Subjects: Q Science > QH Natural history > QH426 Genetics
R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User: Dr. Norafiza Zainuddin
Date Deposited: 12 Oct 2017 16:50
Last Modified: 17 Jan 2018 15:57
URI: http://irep.iium.edu.my/id/eprint/58746

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