Investigation on plasmid DNA separation under vacuum suction using cellulose generated and polyethersulfone ultra filters

Tengku Abdul Hamid, Tengku Haziyamin and Abdul Hamid, Azzmer Azzar (2017) Investigation on plasmid DNA separation under vacuum suction using cellulose generated and polyethersulfone ultra filters. Journal of Applied Pharmaceutical Science, 7 (7). pp. 46-50. ISSN 2231-3354

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Abstract

E. coli DH5α harbouring pET28a+ plasmid vector was lysed by alkaline lysis and the clarified plasmid solutions were subjected to ultrafiltration experiments using two types of ultrafiltration membrane; i.e., cellulose generated (Ultracel) and polyethersulfome PES (Biomax). Transmembrane pressures (TMP) through vacuum suction of 0.1 to 0.7 bar were applied to the plasmid sample, and the corresponding flow rates and fluxes for both filters were investigated. Even though, these two filters showed a slight different in the flux, a marked different in DNA transmission were observed. DNA transmission were generally higher with cellulose generated filters, whereby DNA transmission by Ultracel filter was at44.3, 63.9, 74.1, 55.6 and 54.5% at TMP of 0.1, 0.18, 0.39, 0.6 and 0.7 bar, respectively. Meanwhile, for PES filters the DNA transmission was at 22.3, 38.1, 39.1, 38.0 and 37.5% at TMP of 0.1, 0.3, 0.4, 0.6 and 0.7 bar, respectively. With the cellulose generated filter, DNA transmission reached an optimum (~70%) at about 0.6 bar after which the transmission depleted at higher TMP of 0.7 bar. Throughout all of the TMP, DNA transmissions observed were generally lower with PES filter. The properties of the filter material could have contributed to the differences in DNA permeation

Item Type:	Article (Journal)
Additional Information:	4261/57890
Uncontrolled Keywords:	Plasmid purification, membrane, plasmid flux.
Subjects:	Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Dr Tengku Haziyamin Tengku Abd Hamid
Date Deposited:	08 Aug 2017 12:42
Last Modified:	20 Sep 2017 14:51
URI:	http://irep.iium.edu.my/id/eprint/57890

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