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Optimization of plant growth regulators for Citrus suhuiensis callus induction

M. Fathil, Nur Alia and Mohamad Puad, Noor Illi and Amid, Azura and Azmi, Azlin Suhaida and Ibrahim, Rusli (2017) Optimization of plant growth regulators for Citrus suhuiensis callus induction. Asia-Pacific Journal of Molecular Biology and Biotechnology, 25 (1). pp. 1-7. ISSN 0128-7451

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Correct types and concentration of plant growth regulators (PGRs) will enhance and optimize the growth of callus cultures. This paper reported the effects of several types of cytokinins (2,4-dichlorophenoxyacetic acid (2,4-D) and 1-naphthalene acetic acid (NAA)) and auxins (6–Benzylaminopurine (BAP) and kinetin) on the callus induction of C. suhuiensis. The cotyledons from C. suhuiensis seeds were excised as the explant and cultured on Murashige and Skoog (MS) media, 3% (w/v) sugar, 0.05% (w/v) malt extract and 0.25% (w/v) agar under the continuous dark condition supplemented with the chosen PGRs at the concentration range of 0.5, 1.0, 2.0, 3.0 and 4.0 mg/L. The growth of callus at each treatment was measured as gram (g) of fresh weight and percentage of callus induction. The results showed that 1.0 mg/L 2,4-D gave the highest growth of callus (0.15 g and 100% callus percentage). After identifying the effective PGRs, Central Composite Design (CCD) from the Design Expert® software version 9.0 was used to obtain the optimum concentration of cytokinin and auxin on C. suhuiensis callus cultures. It was observed that the highest amount of callus culture induced was 0.218 g when the media was supplemented with 1.0 mg/L 2,4-D and 1.0 mg/L kinetin.

Item Type: Article (Journal)
Additional Information: 5462/57698
Uncontrolled Keywords: Central composite design (CCD), citrus suhuiensis, callus culture induction, plant growth regulators (PGRs)
Subjects: Q Science > Q Science (General)
T Technology > T Technology (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Noor Illi Mohamad Puad
Date Deposited: 27 Jul 2017 09:07
Last Modified: 01 Feb 2018 01:31
URI: http://irep.iium.edu.my/id/eprint/57698

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