Isolation, cytotoxic and alpha-glucosidase inhibitory activities of new coumarins from calophyllum incrassatum

Aminudin, Nurul Iman and Ahmad, Farediah and Bakhtiar, M. Taher and Mohamed Zulkifli, Razauden (2018) Isolation, cytotoxic and alpha-glucosidase inhibitory activities of new coumarins from calophyllum incrassatum. In: 34th International Conference on Natural Products 2018 (ICNP 2018), 19th-21st March 2018, Penang, Malaysia. (Unpublished)

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Abstract

Calophyllum is a pan-tropical genus belongs to the Guttiferae family and known by Malay local folks as ‘bintangor’. There has been a continual interest to further investigate the phytochemicals of Calophyllum species since this genus is a rich source of active secondary metabolites such as coumarin, chromanone carboxylic acid and flavonoid which showed anti-HIV, cytotoxicity and antimicrobial properties. In this study, phytochemicals studies on the barks and leaves of Calophyllum incrassatum M.R. Henderson & Wyatt-Smith were carried out. Cold extraction method was employed by using dichloromethane, ethyl acetate and methanol to give the respective crude extracts. Four new 4-substituted coumarins, namely, incrassamarin A, B, C and D were successfully isolated from the dichloromethane extracts of the barks and leaves by size-exclusion recycling HPLC and column chromatography techniques. The structures of the compounds were elucidated by spectral analysis including NMR, IR, MS and X-ray crystallography. The compounds were tested for their cytotoxic activity towards human breast adenocarcinoma (MCF-7) and human non-small cell lung carcinoma (A-549) cell lines by MTT assay and α-glucosidase enzymatic inhibitory activity. Incrassamarin A displayed cytotoxic activity against A-549 cell lines with IC50 87.71 mg/ml and showed inhibition towards α-glucosidase enzymatic activity with IC50 93.25 mM.

Item Type:	Conference or Workshop Item (Speech/Talk)
Additional Information:	5209/57430
Uncontrolled Keywords:	Guttiferae; Calophyllum incrassatum; coumarin; cytotoxic; α-glucosidase
Subjects:	R Medicine > RS Pharmacy and materia medica > RS192 Materia Medica-Pharmaceutical Technology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Technology Kulliyyah of Science Kulliyyah of Science > Department of Chemistry
Depositing User:	Dr Muhammad Taher
Date Deposited:	24 Apr 2018 16:42
Last Modified:	30 Jan 2019 12:24
URI:	http://irep.iium.edu.my/id/eprint/57430

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