Phytochemical analysis, identification and quantification of antibacterial active compounds in betel leaves, piper betle methanolic extract

A., Syahidah and Saad, Che Roos and Hassan, M. D. and Rukayadi, Yaya and Mohd Hassan, Norazian and Mohd Salleh, Kamarudin (2017) Phytochemical analysis, identification and quantification of antibacterial active compounds in betel leaves, piper betle methanolic extract. Pakistan Journal of Biological Sciences, 20 (2). pp. 70-81. ISSN 1028-8880

	PDF - Published Version Restricted to Registered users only Download (377kB) | Request a copy
	PDF (SCOPUS) - Supplemental Material Restricted to Repository staff only Download (72kB) | Request a copy

Abstract

Abstract: Background and Objective: The problems of bacterial diseases in aquaculture are primarily controlled by antibiotics. Medicinal plants and herbs which are seemed to be candidates of replacements for conventional antibiotics have therefore gained increasing interest. Current study was performed to investigate the presence of phytochemical constituents, antibacterial activities and composition of antibacterial active compounds in methanolic extract of local herb, Piper betle . Methodology: Qualitative phytochemical analysis was firstly carried out to determine the possible active compounds in P. betle leaves methanolic extract. The antibacterial activities of major compounds from this extract against nine fish pathogenic bacteria were then assessed using TLC-bioautography agar overlay assay and their quantity were determined simultaneously by HPLC method. Results: The use of methanol has proved to be successful in extracting numerous bioactive compounds including antibacterial compounds. The TLC-bioautography assay revealed the inhibitory action of two compounds which were identified as hydroxychavicol and eugenol. The $-caryophyllene however was totally inactive against all the tested bacterial species. In this study, the concentration of hydroxychavicol in extract was found to be 374.72±2.79 mg g–1, while eugenol was 49.67±0.16 mg g–1. Conclusion: Based on these findings, it could be concluded that hydroxychavicol and eugenol were the responsible compounds for the promising antibacterial activity of P. betle leaves methanolic extract. This inhibitory action has significantly correlated with the amount of the compounds in extract. Due to its potential, the extract of P. betle leaves or it compounds can be alternative source of potent natural antibacterial agents for aquaculture disease management.

Item Type:	Article (Journal)
Additional Information:	4171/57315
Uncontrolled Keywords:	Piper betle, methanolic extract, phyto-constituents, antibacterial activity, phenolic compounds, hydroxychavicol, eugenol, TLC, HPLC, quantification, aquaculture
Subjects:	R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy
Depositing User:	Dr. Norazian Mohd Hassan
Date Deposited:	23 Jun 2017 12:07
Last Modified:	28 Feb 2018 09:29
URI:	http://irep.iium.edu.my/id/eprint/57315

Actions (login required)

View Item