Optimization of signalling biomarkers in detecting male infertility

Zahri, Syazana and Abdul Rahman, Suzanah and Abdul Majid, Zafri Azran and Ahmad, Nur Amalina and Abdul Samat, Nadia Hanis and Abdullah, 'Afif Raihan (2016) Optimization of signalling biomarkers in detecting male infertility. In: KAHS Research Week (KRW) 2016 in conjunction with 1st Allied Health Scientific Colloquium (AHSC) 2016, 21st - 25th November 2016, Kuantan, Pahang. (Unpublished)

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Abstract

Objectives/Research Problem: To detect potential signals of fertility biomarkers from non-invasive samples in male reproductive function after X-ray and light exposure to Sprague-Dawley rats. Materials and Method: Male Sprague-Dawley rats with each group (n=6) were exposed to whole body irradiation to high (2.5Gy), medium (0.2Gy) and low (0.05Gy) doses of X-ray. Another group were exposed to continuous light to induce stress. Positive control group were treated with cisplatin and corticosterone while negative control group was left with no intervention. Sampling of blood, saliva and urine will be done prior to exposure and 56 days after exposure. These hormones level in the samples was measured using enzyme-linked immunosorbent assay (ELISA) kit. Histological assessment of the testes and spermatozoa quality were assessed using haematoxylin and eosin (H&E) stain and spermatozoa quality such as sperm count and sperm motility were done together with acridine orange staining to assess DNA. Using the saliva and urine samples, specific signals were captured by transducers, amplified and processed to capture data that reflects the hormone levels. The signals were compared and optimized to produce sensitive tools. Results and Discussion: The expected results was that the X-ray and prolonged light exposure can affect testosterone level that showed reduced man fertility. The presence of this biomarkers in urine and saliva samples can be used to develop diagnostic device/kit. Conclusion: Development of biosensor using significant biomarkers in potentially non-invasive samples can be used in management of infertility.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	4493/56844
Uncontrolled Keywords:	biomarkes, signal,male reproduction, fertility
Subjects:	R Medicine > RG Gynecology and obstetrics R Medicine > RM Therapeutics. Pharmacology R Medicine > RM Therapeutics. Pharmacology > RM695 Physical medicine. Physical therapy
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Allied Health Sciences > Department of Diagnostic Imaging and Radiotherapy
Depositing User:	Prof. Dr. Suzanah Abdul Rahman
Date Deposited:	17 May 2017 09:01
Last Modified:	17 May 2017 09:01
URI:	http://irep.iium.edu.my/id/eprint/56844

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