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Identification of VASA gene expression in in-vitro culture from Non-Obstructive Azoospermia (NOA) testicular biopsy cells: a study to unlock knowledge of male infertility

Md Isa, Muhammad Lokman and Mat Yusof, Afzan and Abdul Wahab, Azantee Yazmie and Ramli, Roszaman (2016) Identification of VASA gene expression in in-vitro culture from Non-Obstructive Azoospermia (NOA) testicular biopsy cells: a study to unlock knowledge of male infertility. In: 2nd World Congress on Integration and Islamicisation: Focus on Medical and Health Care Sciences (2WCII 2016), 21st-23rd Oct. 2016, Kuantan, Pahang Darul Makmur.

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Abstract

The expression product of VASA gene is widely conserved germ line marker and participates in regulating the development of reproductive system and spermatogenesis in healthy man. Azoospermic is a condition which cannot produce sperm cells for reproduction activity. In Islam, seeking knowledge is an obligatory to each Muslim in order to solve problems and for a better live. Thus to unveiled the problem of azoospermic in infertile man we attempt to determine the gene expression of VASA level in testes cells of non-obstructive azoospermic (NOA) sample. Samples were taken from three NOA patients by testicular sperm extraction (TESE) to obtain testicular biopsyies. Testicular cells were isolated and cultured in supplemented knockout DMEM media. VASA gene expression was determined by reverse transcriptase polymerase chain reaction (RT-PCR) for spermatogonial marker. No VASA expression was detected in spermatogonial-like stem cells culture on day 1, 7, 14 and 21. Our finding shown VASA gene was not expressed in vitro culture spermatogenesis might be associated with the abnormal differentiation of primordial germ cells that lead to male infertility.

Item Type: Conference or Workshop Item (Plenary Papers)
Additional Information: 5041/56831
Uncontrolled Keywords: VASA; non-obstructive azoospermia; spermatogenesis; testicular cells; RT-PCR
Subjects: Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Medicine > Department of Obstetric & Gynecology
Kulliyyah of Nursing > Department of Basic Medical Sciences for Nursing
Depositing User: Dr. Afzan Mat Yusof
Date Deposited: 18 May 2017 10:01
Last Modified: 16 Oct 2017 01:01
URI: http://irep.iium.edu.my/id/eprint/56831

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