The effect of particle size, methods and time of extraction on the natural antioxidant content and radical scavenging activity of Phyllanthus niruri

Muhamad Bunnori, Noraslinda and Darnis, Deny Susanti and Yusof, Muhamad Najib and Abd Halim, Khairul Bariyyah (2016) The effect of particle size, methods and time of extraction on the natural antioxidant content and radical scavenging activity of Phyllanthus niruri. In: Medical Research Symposium & Pacific Partnership in conjunction with Kuantan Research Day 2016, 4th April 2016, Kuantan, Pahang. (Unpublished)

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Abstract

Introduction: Phyllanthus niruri has high antioxidant capacity due to high phenolic and flavonoid content. This study is to determine and evaluate the effect of particle size, methods and time of extraction on its antioxidant activities. Methods: P. niruri was dried overnight in drier at 40˚C and grounded to different particle sizes. The extraction methods involved were soxhlet and maceration. Three types of biochemical assays were conducted: total phenolic content (TPC), total flavonoids content (TFC) and 1,1-Diphenyl-2 picrylhydrazyl (DPPH) radical scaveging activity assay. Result: The best TPC and DPPH value is recorded for the sample of less than 0.7 mm, treated by soxhlet extraction method at 8 hours extraction time with TPC valued at 4.95 ± 0.803 mg GAE/g of dried extract and IC50 value of 0.403 ± 0.03790 mg/mL respectively. The highest TFC amount is recorded for the sample with less than 0.7 mm particle size, by maceration method of extraction at 8 hours extraction time with the total flavonoid content of 56.43 ± 0.594 mg QE/g of dried extract. Conclusion: It can be concluded that there are a synergistic effect between TPC and TFC on the quality of the anti-radical property of the sample affecting DPPH activity within the sample.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	6604/56714
Uncontrolled Keywords:	Phyllanthus niruri, antioxidant, total phenolic content, total flavonoids content, 1,1-Diphenyl-2 picrylhydrazyl
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Dr. Noraslinda Muhamad Bunnori
Date Deposited:	03 May 2017 11:59
Last Modified:	03 May 2017 12:07
URI:	http://irep.iium.edu.my/id/eprint/56714

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