The role of DSPF, the type III secretion chaperone of DspA/E of Erwinia amylovora, in pathogenicity in host and non-host plants

Aksoy, Hasan Murad and Kaya, Yilmaz and Tengku Abdul Hamid, Tengku Haziyamin (2017) The role of DSPF, the type III secretion chaperone of DspA/E of Erwinia amylovora, in pathogenicity in host and non-host plants. The Journal of Animal & Plant Sciences, 27 (1). pp. 309-315. ISSN 1018-7081

	PDF - Published Version Restricted to Repository staff only Download (152kB) | Request a copy
	PDF (SCOPUS) - Published Version Restricted to Repository staff only Download (142kB) | Request a copy

Abstract

Erwinia amylovora is a Gram-negative enterobacterium that causes a devastating blight disease on rosaceous plants. In this study, the structure of DspF, a type III secretion system (T3SS) chaperone required for virulence of E. amylovora, was investigated in bacterial growth and pathogenesis in host and non-host plants, including apple, strawberry, rose, Arabidopsis and tobacco. DspF genes were mutated through homologous recombination and the result was the arrest of disease development on apple, strawberry and rose, and of the hypersensitivity response in tobacco. In addition, bacterial development of the wild type was compared with that of dspF mutants in leaves of A. thaliana Columbia-5 (Col-5), and the resistant A. thaliana Landsberg erecta (Ler-0). It was concluded that DspF is essential for the bacterium to induce hypersensitive responce (HR) disease on host plants (Rosaceous family plant) and non-host plants (Arabidopsis thaliana).

Item Type:	Article (Journal)
Additional Information:	4261/56080
Uncontrolled Keywords:	Erwinia amylovora, dspA/E, dspF, pathogenesis
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Dr Tengku Haziyamin Tengku Abd Hamid
Date Deposited:	14 Apr 2017 11:07
Last Modified:	18 Jul 2017 14:29
URI:	http://irep.iium.edu.my/id/eprint/56080

Actions (login required)

View Item