Fabrication and characterization of three-dimensional poly(lactic acid-co-glycolic acid), atelocollagen, and fibrin bioscaffold composite for intervertebral disk tissue engineering application

Mohamad, Mohd Yusof and Mohamed Amin, Muhammad Azri Ifwat and Harun Ismail, Ahmad Fahmi and Md Nazir, Noorhidayah and Ahmad Radzi, Muhammad Aa'zamuddin and Hashim, Rosyafirah and Mat Nawi, Nur Farhana and Zainol, Ismail and Zulkifly, Ahmad Hafiz and Sha'ban, Munirah (2017) Fabrication and characterization of three-dimensional poly(lactic acid-co-glycolic acid), atelocollagen, and fibrin bioscaffold composite for intervertebral disk tissue engineering application. Journal of Bioactive and Compatible Polymers. pp. 1-13. ISSN 0883-9115 E-ISSN 1530-8030 (In Press)

	PDF (Online version full article) - Accepted Version Restricted to Repository staff only Download (640kB) | Request a copy
Preview	PDF (SCOPUS) - Supplemental Material Download (248kB) | Preview
	PDF (Evidence from publishers' website for MYRA) - Published Version Restricted to Repository staff only Download (211kB) | Request a copy

Abstract

The use of synthetically derived poly(lactic-co-glycolic acid) scaffold and naturally derived materials in regeneration of intervertebral disks has been reported in many previous studies. However, the potential effect of poly(lactic-co-glycolic acid) in combination with atelocollagen or fibrin or both atelocollagen and fibrin bioscaffold composite have not been mentioned so far. This study aims to fabricate and characterize three-dimensional poly(lactic-co-glycolic acid) scaffold incorporated with (1) atelocollagen, (2) fibrin, and (3) both atelocollagen and fibrin combination for intervertebral disk tissue engineering application. The poly(lactic-co-glycolic acid) without anynatural, bioscaffold composites was used as control. The chemical conformation, morphology, cell–scaffold attachment, porosity, water uptake capacity, thermal properties, mechanical strength, and pH level were evaluated on all scaffolds using attenuated total reflectance Fourier transform infrared, scanning electron microscope, gravimetric analysis, swelling test, differential scanning calorimetry, and Instron E3000, respectively. Biocompatibility test was conducted to assess the intervertebral disk, annulus fibrosus cells viability using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay. The attenuated total reflectance Fourier transform infrared results demonstrated notable peaks of amide bond suggesting interaction of atelocollagen, fibrin, and both atelocollagen and fibrin combination into the poly(lactic-co-glycolic acid) scaffold. Based on the scanning electron microscope observation, the pore size of the poly(lactic-co-glycolic acid) structure significantly reduced when it was incorporated with atelocollagen and fibrin. The poly(lactic-co-glycolic acid)–atelocollagen scaffolds demonstrated higher significant swelling ratios, mechanical strength, and thermal stability than the poly(lactic-co-glycolic acid) scaffold alone. All the three bioscaffold composite groups exhibited the ability to reduce the acidic poly(lactic-co-glycolic acid) by-product. In this study, the biocompatibility assessment using the 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide cells proliferation assay demonstrated a significantly higher annulus fibrosus cells viability in poly(lactic-co-glycolic acid)–atelocollagen–fibrin compared to poly(lactic-co-glycolic acid) alone. The cellular attachment is comparable in poly(lactic-co-glycolic acid)–atelocollagen–fibrin and poly(lactic-co-glycolic acid)–fibrin scaffolds. Overall, these results may suggest potential use of poly(lactic-co-glycolic acid) combined with atelocollagen and fibrin bioscaffold composite for intervertebral disk regeneration.

Item Type:	Article (Journal)
Additional Information:	2397/55723
Uncontrolled Keywords:	Poly(lactic-co-glycolic acid), atelocollagen, fibrin, intervertebral disk, annulus fibrosus
Subjects:	T Technology > TA Engineering (General). Civil engineering (General) > TA164 Bioengineering
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Medicine > Department of Department of Orthopaedics, Traumatology & Rehabilitation
Depositing User:	Munirah Sha'ban
Date Deposited:	13 Feb 2017 17:03
Last Modified:	21 Mar 2018 08:12
URI:	http://irep.iium.edu.my/id/eprint/55723

Actions (login required)

View Item