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Assessment of insecticide poisoning among vector control operators using a new biomarker, the enyzme β-glucuronidase

Rostam, Muhamad Ashraf and Hassan, Zuridah (2011) Assessment of insecticide poisoning among vector control operators using a new biomarker, the enyzme β-glucuronidase. In: UiTM Sarawak Conference 2011, Kuching, Sarawak.

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The increase of insecticide space spraying to control Aedes mosquitoes submits vector control operators to low-level exposure to toxic organophosphates. Plasma β-glucuronidase level is found to increase in most organophosphate poisoning cases. This study aims to identify presence of organophosphate poisoning using β-glucuronidase assay as an alternative biomarker. A total of 180 blood samples (74 controls and 106 subjects) were examined for this enzyme using a spectrophotometer. These results were then compared to the routine screening assay, butyrylcholinesterase (BChE) assay using a chemical analyzer. Both results showed absence of organophosphate poisoning indicated by no increased in β-glucuronidase level with no reduced in BChE level. Results of BChE level in the subject group (7879.70 ±227.935 IU/L), was significantly higher than the control group (7067.86 ±1609.712 IU/L) (p<0.05). Meanwhile, β- glucuronidase level between the subject (194.48 ±114.711 U/dL) and control group (192.60 ±122.685 U/dL) showed no significant difference (p>0.05). The findings of this evaluation show that assaying for the β-glucuronidase enzyme can be an alternative method to determine organophosphate poisoning. Furthermore, β-glucuronidase assay using spectrophotometer is recommended due to its ease of use and suitability for small laboratories with limited facilities.

Item Type: Conference or Workshop Item (Plenary Papers)
Additional Information: 7744/55399
Uncontrolled Keywords: organophosphate poisoning, β-glucuronidase, cholinesterase assay, alternative biomarker
Subjects: R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences > Department of Nutrition Sciences
Depositing User: Dr Muhamad Ashraf Rostam
Date Deposited: 20 Apr 2017 09:59
Last Modified: 20 Apr 2017 09:59
URI: http://irep.iium.edu.my/id/eprint/55399

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