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Comparison of Trichoderma reesei Rut C-30 and locally isolated Aspergillus spp. cellulases using domestic wastewater sludge as a major substrate

Alam, Md. Zahangir and Mansor, Mariatul F. and Drani , Afifah and Muyibi, Suleyman Aremu and Abdul Karim, Mohamed Ismail and Hamzah, Mohd. Salleh and Kabbashi, Nassereldeen Ahmed (2008) Comparison of Trichoderma reesei Rut C-30 and locally isolated Aspergillus spp. cellulases using domestic wastewater sludge as a major substrate. In: The Sixth International Symposium on Southeast Asian Water Environment, 29 - 31 October 2008, Bandung, Indonesia.

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Abstract

A lab scale study was carried out to produce cellulase enzyme using two types of media: Media B consist of domestic wastewater sludge (DWS), microgranullar cellulose and oil palm empty fruit bunch (EFB) and Media M consist of DWS, microgranullar cellulose, oil palm EFB and addition of methanol. The 3% (v/v) inoculum of Trichoderma reesei Rut C-30 (ATCC 56765) and locally isolated Aspergillus spp. O-102A was inoculated to both media. The maximum total cellulase activity produced was 0.284 FPU/ml (Day 5) and endo-β-1,4 glucanase (CMCase) activity 2.631 IU/ml (Day 7) by T.reesei in media B. The cellulase of T.reesei was higher than O-102A, which only 0.093 FPU/ml (total cellulase activity) and 0.576 IU/ml (CMCase activity) produced by O-102A on the same day of fermentation in media B. The addition of methanol to the substrate (media M) was showing no positive effect to the production of cellulase enzyme for both strains.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 4157/5523
Uncontrolled Keywords: Total cellulase activity, Endo-β-1,4 glucanase activity, Trichoderma reesei, Aspergillus spp., Domestic sewage sludge
Subjects: T Technology > TP Chemical technology
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr. Md. Zahangir Alam
Date Deposited: 08 Aug 2012 15:07
Last Modified: 08 Aug 2012 15:07
URI: http://irep.iium.edu.my/id/eprint/5523

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