Md Nazir, Noorhidayah and Zulkifly, Ahmad Hafiz and Khalid, Kamarul Ariffin and Zainol, Ismail and Zamli, Zaitunnatakhin and Sha'ban, Munirah
(2016)
In vitro and in vivo non-viral SRY (Sex Determining Region Y)-box 9 (SOX9) and telomerase reverse transcriptase (TERT) genes transfer in chondrocytes: work in progress.
In: KAHS Research Week (KRW) 2016 in conjunction with 1st Allied Health Scientific Colloquium (AHSC) 2016, 21 - 25 November 2016, Kuantan.
Abstract
Objectives/Research Problem: Degenerative joint disease normally affects older population which
eventually disturb patient’s mobility. Current available prescriptions and surgical procedures are
incapable to cure the disease completely due to the nature of avascular cartilage. Gene transfer and tissue
engineering have emerged as promising approaches in treating the disease. The idea behind the
incorporation of the two approaches is to regenerate a stable tissue engineered cartilage constructs by
enhancing the cells source. Hence, the aim of this study is to assess the effect of non-viral SOX9 and
TERT genes transfected chondrocytes in vitro and in vivo.
Materials and Method: With the approval of Institutional Animal Care and Use Committee (IACUCIIUM)
(IIUM/IACUC/Approval 2015/(5)/(24)), SOX9 and TERT genes are transferred in monolayer
cultured chondrocytes via lipofection technique. The post-transfected chondrocytes are then seeded in a
three-dimensional (3D) poly(lactic-co-glycolic) (PLGA) with and without fibrin scaffolds for tissue
engineered cartilage constructs formation in vitro. The resulting constructs are implanted subcutaneously
in the athymic nude mice for constructs’ maturation purpose. The incorporation of cells in the 3D scaffold
in vitro and the in vivo implantation are important to mimic the internal microenvironment of the human
body. The monolayer post-transfected chondrocytes, in vitro and in vivo 3D constructs are evaluated with
several different assessments namely glycosaminoglycan (GAG) assay, cell proliferation assay,
histological and immunohistochemistry, gene expression assessment. This is done to confirm the
chondrogenic properties of the constructs based on selected time points.
Results and Discussion: Several preliminary findings are obtained. The results show certain trend towards
synergistic effect of both SOX9 and TERT genes on chondrocytes. However, no conclusion can be made
at this juncture. The experiment is still ongoing.
Conclusion: It is hoped that the combination of SOX9 and TERT genes can help regenerate a good
quality cartilage for tissue engineering purpose.
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